Wiedenmann B, Franke W W, Kuhn C, Moll R, Gould V E
Proc Natl Acad Sci U S A. 1986 May;83(10):3500-4. doi: 10.1073/pnas.83.10.3500.
Synaptophysin is an integral membrane glycoprotein (Mr 38,000) that occurs in presynaptic vesicles of neurons and in similar vesicles of the adrenal medulla. By using a monoclonal antibody to this protein (SY38), we have found, by immunohistochemistry and immunoblotting, that an identical or similar protein is also expressed in neuroendocrine tumors of neural type, such as pheochromocytomas and paragangliomas. In addition, this protein occurs in certain neuroendocrine epithelial cells, such as pancreatic islet cells; in a variety of neuroendocrine epithelial tumors, including isletcell adenomas and carcinomas and several carcinoids and neuroendocrine carcinomas of the gastrointestinal and the bronchial tracts; and in medullary carcinomas of the thyroid. Our results show that synaptophysin, and the vesicles that contain it, can occur in normal and neoplastic neuroendocrine cells of neural type, as demonstrated by colocalization with neurofilaments, as well as in those of epithelial type, as shown by colocalization with cytokeratin filaments and desmoplakins. We conclude that synaptophysin is expressed independently of other neuronal differentiation markers and propose that it be used as a differentiation marker in tumor diagnosis.
突触素是一种整合膜糖蛋白（分子量38,000），存在于神经元的突触前囊泡以及肾上腺髓质的类似囊泡中。通过使用针对该蛋白的单克隆抗体（SY38），我们通过免疫组织化学和免疫印迹发现，在神经型神经内分泌肿瘤，如嗜铬细胞瘤和副神经节瘤中也表达相同或相似的蛋白。此外，这种蛋白存在于某些神经内分泌上皮细胞中，如胰岛细胞；存在于多种神经内分泌上皮肿瘤中，包括胰岛细胞腺瘤和癌以及胃肠道和支气管的几种类癌和神经内分泌癌；还存在于甲状腺髓样癌中。我们的结果表明，突触素及其包含的囊泡可存在于神经型正常和肿瘤性神经内分泌细胞中，这通过与神经丝共定位得以证明，也可存在于上皮型细胞中，这通过与细胞角蛋白丝和桥粒斑蛋白共定位得以显示。我们得出结论，突触素的表达独立于其他神经元分化标志物，并建议将其用作肿瘤诊断中的分化标志物。
