Kreitman M, Aguadé M
Proc Natl Acad Sci U S A. 1986 May;83(10):3562-6. doi: 10.1073/pnas.83.10.3562.
A filter hybridization method is described for identifying restriction-site and insertion/deletion variation by using restriction enzymes that recognize four-nucleotide sequences and denaturing polyacrylamide gels for separating fragments. Eighty-seven lines of Drosophila melanogaster representing two natural populations were surveyed over a 2.7-kilobase region encompassing the alcohol dehydrogenase locus. Fifty distinct haplotypes were identified from 17 restriction-site and 11 insertion/deletion polymorphisms and from one allozyme polymorphism. There was no evidence for genetic differentiation between an East-Coast and a West-Coast (North American) sample. This technique has widespread applications in screening for DNA polymorphism.
描述了一种滤膜杂交方法，该方法通过使用识别四核苷酸序列的限制性内切酶和用于分离片段的变性聚丙烯酰胺凝胶来鉴定限制性位点以及插入/缺失变异。在包含乙醇脱氢酶基因座的2.7千碱基区域内，对代表两个自然种群的87条黑腹果蝇品系进行了调查。从17个限制性位点、11个插入/缺失多态性以及1个等位酶多态性中鉴定出50种不同的单倍型。没有证据表明东海岸和西海岸（北美）样本之间存在遗传分化。这项技术在筛选DNA多态性方面有广泛的应用。
