Laboratory of Process Engineering, Faculty of Technology, Laghouat University, BP 37 G Ghardaia Road, 03000 Laghouat, Algeria.
Centro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, Portugal.
Molecules. 2018 Aug 10;23(8):1994. doi: 10.3390/molecules23081994.
The present study focuses on the chemical composition, antioxidant, antimicrobial, and antiproliferative activities of the ethyl acetate and aqueous extracts obtained from the aerial parts of Pourr. HPLC-DAD-ESI/MSn was used to identify the phenolic compounds, being (epi)gallocatechin dimer the major compound (111 ± 5 µg/g·dw) in the aqueous extract, while myricetin--rhamnoside (226 ± 9 µg/g·dw) was the main molecule in the ethyl acetate extract. Both extracts exhibited good scavenging activities against DPPH radical (EC µg/mL of 59 ± 2 and 31 ± 2 for aqueous and ethyl acetate extracts, respectively). However, the ethyl acetate extract demonstrated more potent quenching activities than the aqueous extract. The antimicrobial activities were assessed on selected Gram-positive () and Gram-negative ( and ) bacteria, as well as on pathogenic fungus . The extracts possessed selective and potent antimicrobial activities against the Gram-positive bacterium (IC of 186 ± 9 μg/mL and 92 ± 3 μg/mL for aqueous and ethyl acetate extracts, respectively). Finally, extracts were evaluated for their antiproliferative potential on three human cancer cell lines representing breast and colon cancers. Although both extracts demonstrated sufficient growth inhibition of the three different cell lines, the ethyl acetate extract exhibited higher activity (LD values of 1.57 ± 0.06 mg/mL, 2.2 ± 0.1 mg/mL, and 3.2 ± 0.2 mg/mL for T47D, MCF-7, and HCT-116 cell lines). Both the extracts obtained from the aerial parts of revealed very promising results and could be applied as functional agents in the food, pharmaceutical, and cosmeceutical industries.
本研究重点关注从 Pourr 地上部分获得的乙酸乙酯和水提物的化学成分、抗氧化、抗菌和抗增殖活性。HPLC-DAD-ESI/MSn 用于鉴定酚类化合物，水提物中的主要化合物为（表）没食子儿茶素二聚体（111 ± 5 µg/g·dw），而乙酸乙酯提取物中的主要分子为杨梅素-鼠李糖苷（226 ± 9 µg/g·dw）。两种提取物均表现出良好的 DPPH 自由基清除活性（EC µg/mL 值分别为 59 ± 2 和 31 ± 2）。然而，乙酸乙酯提取物的淬灭活性强于水提物。抗菌活性评估了选定的革兰氏阳性（）和革兰氏阴性（和）细菌以及致病性真菌。提取物对革兰氏阳性菌具有选择性和强效的抗菌活性（IC 分别为 186 ± 9 µg/mL 和 92 ± 3 µg/mL）。最后，评估了 提取物对三种人癌细胞系（代表乳腺癌和结肠癌）的抗增殖潜力。尽管两种提取物均显示出对三种不同细胞系的足够生长抑制作用，但乙酸乙酯提取物表现出更高的活性（LD 值分别为 1.57 ± 0.06 mg/mL、2.2 ± 0.1 mg/mL 和 3.2 ± 0.2 mg/mL，用于 T47D、MCF-7 和 HCT-116 细胞系）。从 Pourr 地上部分获得的两种提取物均显示出非常有前景的结果，可作为食品、制药和化妆品行业的功能性制剂应用。
