Laboratory of Biomolecules, Venoms and Theranostic Applications, LR20IPT01, Institut Pasteur of Tunis, University of Tunis El Manar, Tunis 1002, Tunisia.
Laboratory of Management and Valorization of Forest Resources, National Research Institute of Rural Engineering, Water and Forestry (INRGREF), University of Carthage, Tunis 1004, Tunisia.
Molecules. 2022 Jan 5;27(1):314. doi: 10.3390/molecules27010314.
Mint species ( family) have been used as traditional remedies for the treatment of several diseases. In this work, we aimed to characterize the biological activities of the total phenolic and flavonoid contents of L. extracts collected from two different regions of Tunisia. The highest amounts of total phenols (74.45 ± 0.01 mg GAE/g DW), flavonoids (28.87 ± 0.02 mg RE/g DW), and condensed tannins (4.35 ± 0.02 mg CE/g DW) were found in the Bizerte locality. Methanolic leaf extracts were subjected to HPLC-UV analysis in order to identify and quantify the phenolic composition. This technique allowed us to identify seven phenolic compounds: two phenolic acids and five flavonoid compounds, such as eriocitrin, hesperidin, narirutin, luteolin, and isorhoifolin, which were found in both extracts with significant differences between samples collected from the different regions ( < 0.05). Furthermore, our results showed that the methanolic extract from leaves collected from Bizerte had the highest antioxidant activities (DPPH IC value of 16.31 μg/mL and 570.08 μmol Fe/g, respectively). Both extracts showed high radical-scavenging activity as well as significant antimicrobial activity against eight tested bacteria. The highest antimicrobial activities were observed against Gram-positive bacteria with inhibition zone diameters and MIC values ranging between 19 and 32 mm and 40 and 160 µg/mL, respectively. Interestingly, at 10 μg/mL, the extract had a significant effect on cell proliferation of U87 human glioblastoma cells. These findings open perspectives for the use of L. extract in green pharmacy, alternative/complementary medicine, and natural preventive therapies for the development of effective antioxidant, antibacterial, and/or antitumoral drugs.
薄荷属（family）植物被用作传统疗法，用于治疗多种疾病。在这项工作中，我们旨在描述来自突尼斯两个不同地区的 L.提取物的总酚和类黄酮含量的生物活性。在比塞大地区发现了最高量的总酚（74.45 ± 0.01 mg GAE/g DW）、类黄酮（28.87 ± 0.02 mg RE/g DW）和缩合单宁（4.35 ± 0.02 mg CE/g DW）。甲醇叶提取物通过 HPLC-UV 分析进行分析，以鉴定和定量酚类成分。这项技术使我们能够鉴定出七种酚类化合物：两种酚酸和五种类黄酮化合物，如橙皮苷、柚皮苷、柚皮苷、木樨草素和异槲皮苷，它们在两个提取物中均被发现，且在不同地区采集的样品之间存在显著差异（<0.05）。此外，我们的结果表明，来自比塞大地区的叶片甲醇提取物具有最高的抗氧化活性（DPPH IC 值分别为 16.31 μg/mL 和 570.08 μmol Fe/g）。两种提取物均表现出较高的自由基清除活性和对八种测试细菌的显著抗菌活性。对革兰氏阳性菌的抗菌活性最强，抑菌圈直径和 MIC 值分别为 19-32 mm 和 40-160 μg/mL。有趣的是，在 10 μg/mL 时，提取物对 U87 人胶质母细胞瘤细胞的增殖有显著影响。这些发现为 L.提取物在绿色制药、替代/补充医学以及天然预防疗法中的应用开辟了前景，可用于开发有效的抗氧化、抗菌和/或抗肿瘤药物。
