Department of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan, Hubei, China.
Endoscopy Center, Minhang Branch of Zhongshan Hospital, Fudan University, Shanghai, China.
Biomed Res Int. 2018 Jul 12;2018:8973986. doi: 10.1155/2018/8973986. eCollection 2018.
Inhibiting vascular endothelial foam is the focus of clinical attention. Using SonoVue (an ultrasound contrast agent), the salusin- gene was transfected into the arterial intima of an atherosclerotic rabbit model induced by a high-fat diet in this study. Subsequently the model of blood lipid indexes, the pathological structure of the intima, and changes in molecules regulating atherosclerosis were investigated. The high-density lipoprotein C and apolipoprotein A values in the salusin- gene overexpression (P) group were higher than those in the salusin- gene interference (RP) group ( < 0.05), whereas the total cholesterol, low-density lipoprotein C, and apolipoprotein B values were reversed. Rabbits in the P group showed significantly thinner vascular intimal thickness than that of other experimental groups ( < 0.05). The expression of positive regulators of atherosclerosis (, ) was higher in the P group than that in the RP group ( < 0.05), and the opposite effect was observed for negative regulators (, ). Thus, our results showed that the overexpression of salusin- gene inhibited the proliferation of the vascular intima, thereby throwing some light on understanding the mechanism how salusin- gene expression interfered with the foaming of vascular intimal cells.
抑制血管内皮泡沫化是临床关注的焦点。本研究采用高脂饮食诱导的动脉粥样硬化兔模型，应用声诺维（超声造影剂）转染血管内膜的 salusin- 基因，探讨血脂指标模型、内膜病理结构及调控动脉粥样硬化相关分子的变化。salusin- 基因过表达（P）组高密度脂蛋白胆固醇和载脂蛋白 A 值高于 salusin- 基因干扰（RP）组（ < 0.05），而总胆固醇、低密度脂蛋白胆固醇和载脂蛋白 B 值则相反。P 组兔血管内膜厚度明显薄于其他实验组（ < 0.05）。动脉粥样硬化的阳性调节因子（、）在 P 组的表达高于 RP 组（ < 0.05），而阴性调节因子（、）则相反。因此，我们的结果表明，salusin- 基因的过表达抑制了血管内膜的增殖，从而为理解 salusin- 基因表达如何干扰血管内膜细胞泡沫化的机制提供了一些线索。
