Department of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan, China.
Central Laboratory, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
J Ultrasound Med. 2022 Feb;41(2):365-376. doi: 10.1002/jum.15714. Epub 2021 Apr 5.
A new method based on the adhesion of SonoVue to plasmids was assessed to achieve targeted gene delivery into the vascular endothelium.
pEGFP-Salusin-α and pcDNA3.1-Salusin-α plasmids were transfected into the arterial endothelium of different rabbit groups. Western blotting was performed to analyze the expression of EGFP and salusin-α in the common carotid arteries of rabbits from different groups, and ELISA was performed to detect plasma salusin-α levels in rabbits from each group; simultaneously, blood parameters of different groups of rabbits were measured.
Green fluorescence was observed in the right common carotid artery of rabbits transfected with pEGFP-Salusin-α, but not in the endothelial cells of not-transfected control rabbits. The expression of salusin-α in the transfected animals was higher than that in the control not-transfected animals (P < .05). In rabbits transfected with pcDNA3.1-Salusin-α plasmid, salusin-α expression was higher than in the not-transfected control animals (P < .05). However, there was no significant difference in plasma salusin-α levels between transfected animals and controls (P > .05). Blood parameters were also measured in both groups.
Our data confirm the establishment of a new method using SonoVue for targeted gene delivery into the arterial endothelium. Our study outcomes propose a new method of intervention in atherosclerosis and a new tool for targeted gene delivery.
评估一种基于 SonoVue 与质粒结合的新方法，以实现血管内皮的靶向基因传递。
将 pEGFP-Salusin-α 和 pcDNA3.1-Salusin-α 质粒转染到不同兔组的动脉内皮中。通过 Western blot 分析不同组兔颈总动脉中 EGFP 和 salusin-α 的表达，通过 ELISA 检测各组兔血浆 salusin-α 水平；同时，测量各组兔的血液参数。
转染 pEGFP-Salusin-α 的兔右侧颈总动脉可见绿色荧光，但未转染对照组兔的内皮细胞未见荧光。转染动物的 salusin-α 表达高于未转染对照组动物（P<.05）。转染 pcDNA3.1-Salusin-α 质粒的兔 salusin-α 表达高于未转染对照组动物（P<.05）。然而，转染动物与对照组之间的血浆 salusin-α 水平无显著差异（P>.05）。两组均测量了血液参数。
我们的数据证实了使用 SonoVue 靶向基因传递到动脉内皮的新方法的建立。我们的研究结果提出了一种新的干预动脉粥样硬化的方法和一种新的靶向基因传递工具。
