Platt K B, Hill H T, Seymour C L, Pirtle E C
Vet Microbiol. 1986 Feb;11(1-2):25-40. doi: 10.1016/0378-1135(86)90004-0.
An early virus protein complex that is found in the maintenance medium of Aujeszky's disease (AD) virus-infected cells was evaluated as a subunit diagnostic antigen (SUDA) in the enzyme-linked immunosorbent assay (ELISA). This antigen was found in purer form and in larger quantities for up to 12 h post-infection in the maintenance medium of AD virus-infected MDBK cell cultures than in the maintenance medium of virus-infected porcine Fallopian tube (PFT) and PK1a cell cultures. The SUDA was shown to be compatible with a lectin-derived subunit vaccine by the absence of positive ELISA reactions for antibody to this antigen in 25 AD virus-free subunit-vaccinated pigs. Following virus challenge, all fo 24 surviving vaccinated pigs seroconverted to SUDA within 10 days. Compatibility with the vaccine was further demonstrated by the absence of positive ELISA reactions for antibody to SUDA in 12 pigs that received five or six consecutive vaccine doses at 3-wk intervals. The sensitivity of the ELISA with SUDA was demonstrated by the detection of antibody in virus-infected vaccinated and non-vaccinated pigs for at least 15 and 22 weeks, respectively, following exposure to virus. The SUDA was also economical: it was calculated that 8000-14 000 tests could be run with the antigen present in the maintenance medium of one 850 cm2 plastic tissue culture roller bottle of virus-infected MDBK cells.
在酶联免疫吸附测定（ELISA）中，对在感染奥耶斯基氏病（AD）病毒的细胞的维持培养基中发现的一种早期病毒蛋白复合物进行了评估，将其作为亚单位诊断抗原（SUDA）。与感染病毒的猪输卵管（PFT）细胞培养物和PK1a细胞培养物的维持培养基相比，在感染AD病毒的MDBK细胞培养物的维持培养基中，这种抗原在感染后长达12小时内以更纯的形式且大量存在。在25头未感染AD病毒的接种亚单位疫苗的猪中，针对该抗原的抗体未出现ELISA阳性反应，表明SUDA与一种凝集素衍生的亚单位疫苗兼容。在病毒攻击后，所有24头存活的接种疫苗的猪在10天内血清转化为对SUDA呈阳性。在12头以3周间隔连续接种五或六剂疫苗的猪中，针对SUDA的抗体未出现ELISA阳性反应，进一步证明了与疫苗的兼容性。用SUDA进行的ELISA的敏感性通过在感染病毒后分别在接种疫苗和未接种疫苗的猪中至少15周和22周检测到抗体得以证明。SUDA也很经济：据计算，用一个850平方厘米塑料组织培养滚瓶中感染病毒的MDBK细胞的维持培养基中存在的抗原，可以进行8000 - 14000次检测。
