Platt K B
Vet Microbiol. 1982 Dec;7(6):515-34. doi: 10.1016/0378-1135(82)90046-3.
Twenty Aujeszky's disease (AD) virus antigens were demonstrated by crossed immunoelectrophoresis in a Triton-X-100 detergent extract of virus-infected PK-1a cells. Eight of these antigens were shown to be glycosylated based on their ability to be specifically bound by the lectin Ricinus communis agglutinin II. Pigs nasally infected with AD virus showed a significant serum antibody titer to seven of the known glycosylated antigens and to four additional antigens. The antibody titer to these antigens persisted for at least 116 days. Pigs which were vaccinated parenterally with the whole detergent extract survived a nasal challenge of 10(8 . 5) PFU of virulent AD virus. The antibody response of these vaccinated pigs on the day of challenge was essentially identical to the recovery response previously observed in non-vaccinated nasally infected pigs. These results indicate that the optimum components of future AD virus subunit vaccines and their complementary diagnostic reagents should be selected from these 11 antigens.
通过交叉免疫电泳在病毒感染的PK - 1a细胞的Triton - X - 100去污剂提取物中检测到20种奥耶斯基氏病（AD）病毒抗原。基于它们被凝集素蓖麻凝集素II特异性结合的能力，其中8种抗原被证明是糖基化的。经鼻腔感染AD病毒的猪对7种已知的糖基化抗原和另外4种抗原表现出显著的血清抗体效价。对这些抗原的抗体效价持续至少116天。经肌肉注射用整个去污剂提取物进行疫苗接种的猪在受到10(8.5) PFU强毒AD病毒的鼻腔攻毒后存活下来。这些接种疫苗的猪在攻毒当天的抗体反应与先前在未接种疫苗但经鼻腔感染的猪中观察到的恢复反应基本相同。这些结果表明，未来AD病毒亚单位疫苗及其互补诊断试剂的最佳成分应从这11种抗原中选择。
