Liu Lanlan, Zhao Ling, Cheng Ke, Deng Haiping, Guo Menghu, Wei Jianzi, Shen Xueyong
Acupuncture & Tuina College, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China.
Shanghai Research Center of Acupuncture & Meridians, 1200, Cailun RD, Shanghai, 201203, China.
Lasers Med Sci. 2019 Mar;34(2):263-271. doi: 10.1007/s10103-018-2581-y. Epub 2018 Jul 22.
To investigate the cell cycle and cellular mechanisms of leukocyte elevation by laser acupuncture in rats with cyclophosphamide (CTX)-induced leukopenia. Sixty-six rats were randomized into six groups: normal, model control group, sham treatment group, 10.6 μm laser treatment group, 650 nm laser treatment group, and 10.6 μm-650 nm compound laser treatment group. Eleven rats were used in the normal group and 55 were models that were injected with cyclophosphamide to induce leukopenia. For the three laser treatment groups, 10.6-μm and 650-nm lasers, and 10.6-μm-650-nm compound lasers were used to irradiate the DU14 (Dazhui) and bilateral ST36 (Zusanli) for 5 min each. The sham laser group received the same operation as the laser group but without irradiation. The normal group and model group were not treated. Differences in the number of nucleated cells in the femoral bone marrow, and cell cycle and cellular apoptosis of peripheral leukocytes in rats in various groups were compared. Compared with the model group and the sham laser group, the number of nucleated cells in the femoral bone marrow in the 10.6-μm laser, 650-nm laser, and 10.6-μm-650-nm compound laser group was significantly increased after treatment (P = 0.001, 0.002, 0.034, respectively) and did not show any significant difference with the normal group (P = 0.964, P = 0.838, P = 0.287, respectively). The number of cells in G2 phase in the 10.6 μm laser group was similar to that of the normal group (P = 0.973). The number of cells in G2 phase in the model, sham, 650-nm laser group, and 10.6-μm-650-nm compound laser group were significantly lower than in the normal group and 10.6-μm laser group (P = 0.016, P = 0.023, P = 0.044, P = 0.039, respectively). In the model group and the sham treatment group, the apoptosis rates of peripheral leukocytes were increased compared with the normal group (P = 0.001), while the proportion of cells in the G2 phase was significantly lower than in the normal group (P = 0.016), and the proportion of cells in S phase was higher than in the normal group (P = 0.014). The incidence of apoptosis in peripheral blood cells in the three laser treatment groups did not show any statistically significant difference when compared with the normal group (P > 0.05). Treatment with the 10.6-μm, 650-nm, and 10.6-μm-650-nm compound lasers increased the number of nucleated cells in the bone marrow, decreased the unfavorable effects of cyclophosphamide on the cell cycle, induced the cell cycle towards proliferation, decreased apoptosis, improved the intramedullary hematopoietic system, and increased peripheral leukocyte count.
为研究激光针刺对环磷酰胺（CTX）诱导的白细胞减少症大鼠白细胞升高的细胞周期及细胞机制。将66只大鼠随机分为6组：正常组、模型对照组、假治疗组、10.6μm激光治疗组、650nm激光治疗组和10.6μm - 650nm复合激光治疗组。正常组11只大鼠，其余55只大鼠注射环磷酰胺诱导白细胞减少症作为模型。对于三个激光治疗组，分别用10.6μm和650nm激光以及10.6μm - 650nm复合激光照射大鼠的DU14（大椎）和双侧ST36（足三里），每次照射5分钟。假激光组接受与激光组相同的操作但不进行照射。正常组和模型组不进行处理。比较各组大鼠股骨骨髓中有核细胞数量、外周血白细胞的细胞周期及细胞凋亡情况。与模型组和假激光组相比，10.6μm激光组、650nm激光组和10.6μm - 650nm复合激光组治疗后股骨骨髓中有核细胞数量显著增加（P分别为0.001、0.002、0.034），与正常组相比无显著差异（P分别为0.964、0.838、0.287）。10.6μm激光组G2期细胞数量与正常组相似（P = 0.973）。模型组、假治疗组、650nm激光组和10.6μm - 650nm复合激光组G2期细胞数量显著低于正常组和10.6μm激光组（P分别为0.016、0.023、0.044、0.039）。模型组和假治疗组外周血白细胞凋亡率高于正常组（P = 0.001），G2期细胞比例显著低于正常组（P = 0.016），S期细胞比例高于正常组（P = 0.014）。三个激光治疗组外周血细胞凋亡发生率与正常组相比无统计学显著差异（P > 0.05）。10.6μm、650nm和10.6μm - 650nm复合激光治疗可增加骨髓中有核细胞数量，减轻环磷酰胺对细胞周期的不良影响，诱导细胞周期向增殖方向发展，减少细胞凋亡，改善骨髓造血系统，增加外周血白细胞计数。
