1Université Clermont Auvergne, INRA, VetAgro Sup, UMR Herbivores,F-63122 Saint-Genès-Champanelle,France.
2AgroParisTech,INRA,UMR0791 Mosar,16 rue Claude Bernard,F-75231 Paris,France.
Animal. 2019 Apr;13(4):727-739. doi: 10.1017/S1751731118001982. Epub 2018 Aug 14.
Dietary and ruminal factors modify the ruminal biohydrogenation (RBH) of polyunsaturated fatty acids (FA), with duodenal FA flows being quantitatively and qualitatively different from FA intake. Using a meta-analysis approach from a database on duodenal flows of FA in ruminants, this study aimed to determine predictive equations for duodenal and absorbed flows of saturated FA, C18:1, C18:2 and C18:3 isomers, odd- and branched-chain FA (OBCFA), C20:5n-3, C22:5n-3 and C22:6n-3 and to quantify the effects of dietary and digestive factors on those equations. The database was divided into four subsets: forage, seed, vegetable oils or animal fats (oil/fat), and fish products (fish) subsets. Models of duodenal and absorbed FA flows were obtained through variance-covariance analysis. Effects of potential interfering factors (conservation mode and botanical families of forages, lipid source, technological processing of lipid supplements, diet composition and animal characteristics) were analysed. We obtained 83 models for duodenal FA flows as a function of FA intake for saturated FA (C14:0, C16:0 and C18:0), C18:1, C18:2 and C18:3 isomers and seven other models for OBCFA. For the seed/oil/fat subset, intakes of total C18:3, C18:2 and starch content increased the duodenal t11-C18:1 flow with 0.08, 0.16 and 0.005 g/kg of dry matter intake (DMI), respectively, whereas intake level [(DMI×100)/BW] decreased it. The c9c12c15-C18:3 RBH was higher for oil/fat than seed (96.7% v. 94.8%) and a protective effect of Leguminosae v. Gramineae against RBH for that FA appeared in the forage subset. The duodenal C17:0 flow increased with starch content and decreased with ruminal pH, respectively, whereas duodenal iso-C16:0 flow decreased with dietary NDF content for the seed/oil/fat subset. The duodenal C20:5n-3, C22:5n-3 and C22:6n-3 flows depended on their respective intake and the inhibitory effect of C22:6n-3 on duodenal C18:0 flow was quantified. Thirteen models of absorbed FA flows were performed depending on their respective duodenal flows. This study determined the effects of different qualitative and quantitative dietary and digestive factors, allowing for improved predictions of duodenal and absorbed FA flows.
饮食和瘤胃因素会改变多不饱和脂肪酸（PUFA）的瘤胃生物氢化（RBH），十二指肠脂肪酸（FA）流量在数量和质量上与 FA 摄入不同。本研究使用数据库中反刍动物十二指肠 FA 流量的荟萃分析方法，旨在确定预测方程，用于预测饱和 FA、C18:1、C18:2 和 C18:3 异构体、奇数和支链 FA（OBCFA）、C20:5n-3、C22:5n-3 和 C22:6n-3 的十二指肠和吸收流量，并量化饮食和消化因素对这些方程的影响。该数据库分为四个子集：草料、种子、植物油或动物脂肪（油/脂）和鱼类产品（鱼）子集。通过方差协方差分析获得十二指肠和吸收 FA 流量模型。分析了潜在干扰因素（草料的保存方式和植物科、脂质来源、脂质补充剂的技术加工、饮食组成和动物特征）的影响。我们获得了 83 个用于饱和 FA（C14:0、C16:0 和 C18:0）、C18:1、C18:2 和 C18:3 异构体以及其他 7 个用于 OBCFA 的十二指肠 FA 流量的函数模型。对于种子/油/脂子集，总 C18:3、C18:2 和淀粉含量的摄入量分别增加了 0.08、0.16 和 0.005 g/kg 干物质摄入量（DMI）的十二指肠 t11-C18:1 流量，而摄入量水平[（DMI×100）/BW]则降低了它。油/脂的 C9c12c15-C18:3 RBH 高于种子（96.7% v. 94.8%），豆类科对该 FA 的 RBH 具有保护作用，而禾本科则对其具有保护作用，这在草料子集中出现。十二指肠 C17:0 流量随淀粉含量增加而增加，随瘤胃 pH 值降低而降低，而种子/油/脂子集的十二指肠 iso-C16:0 流量随饲粮 NDF 含量降低而降低。十二指肠 C20:5n-3、C22:5n-3 和 C22:6n-3 流量取决于各自的摄入量，并且 C22:6n-3 对十二指肠 C18:0 流量的抑制作用也得到了量化。根据各自的十二指肠流量，进行了 13 个吸收 FA 流量模型。本研究确定了不同定性和定量饮食和消化因素的影响，从而可以更好地预测十二指肠和吸收 FA 流量。
