Gómez-Cortés Pilar, Requena Domenech Francisco, Correro Rueda Marta, de la Fuente Miguel Ángel, Schiavone Achille, Martínez Marín Andrés L
Instituto de Investigación en Ciencias de la Alimentación (CSIC-UAM), Universidad Autónoma de Madrid, Nicolás Cabrera 9, 28049 Madrid, Spain.
Departamento de Biología Celular, Fisiología e Inmunología, Universidad de Córdoba, Ctra. Madrid-Cádiz km 396, 14071 Córdoba, Spain.
Foods. 2021 Jan 3;10(1):77. doi: 10.3390/foods10010077.
There is a growing interest of researchers in meat authentication in terms of geographical and dietary background of animals, and several analytical methods have been proposed for the purpose of investigating this. We hypothesized that the odd- and branched-chain fatty acid (OBCFA) profile in intramuscular fat (IMF) might suffice to distinguish lamb meat entering the food chain supply on the basis of the type of diet fed to lambs during the fattening period. A total of 30 individual OBCFA profiles, quantified by gas chromatography, of IMF of Manchego lambs were used. During the fattening period (42 days), the lambs were fed three diets differing in concentrate composition: (i) Control, concentrate typical of commercial fattening rations, rich in starch and based on cereals and soybean meal; (ii) Camelina, similar to Control but replacing 50% of the soybean meal with camelina meal; and (iii) Fibrous, concentrate rich in neutral detergent fiber (NDF), based on fibrous by-products and not including cereals nor soybean meal. The OBCFA were grouped into three classes (linear odd, iso and anteiso fatty acids) and were then submitted to a linear discriminant analysis, using the feeding treatments as grouping variable and the OBCFA class contents in IMF as quantitative variables. The results suggested that a high NDF to starch ratio of the concentrate, being the lowest for Control (CON) treatment and the highest for Fibrous (FIB) treatment, would be negatively related to the odd/anteiso ratio and positively related to the iso/(anteiso+odd) FA ratio in IMF. Determination of OBCFA profile in lamb meat would be useful to monitor the feeding regime (starch- or NDF-rich) of lambs entering the food chain supply.
研究人员对基于动物地理和饮食背景的肉类认证越来越感兴趣，并且已经提出了几种分析方法来进行这方面的研究。我们假设，肌内脂肪（IMF）中的奇数和支链脂肪酸（OBCFA）谱可能足以根据育肥期羔羊所喂饲料的类型来区分进入食物链供应的羊肉。我们使用了通过气相色谱法定量的30份曼彻格羔羊IMF的个体OBCFA谱。在育肥期（42天），给羔羊喂食三种精料组成不同的日粮：（i）对照日粮，即商业育肥日粮中典型的富含淀粉且以谷物和豆粕为基础的精料；（ii）亚麻荠日粮，与对照日粮相似，但用亚麻荠粕替代50%的豆粕；（iii）纤维日粮，富含中性洗涤纤维（NDF）的精料，以纤维副产品为基础，不包括谷物和豆粕。将OBCFA分为三类（直链奇数、异和反异脂肪酸），然后以饲养处理为分组变量，以IMF中OBCFA类含量为定量变量进行线性判别分析。结果表明，精料中高NDF与淀粉比（对照（CON）处理最低，纤维（FIB）处理最高）与IMF中的奇数/反异比呈负相关，与异/（反异+奇数）脂肪酸比呈正相关。测定羊肉中的OBCFA谱将有助于监测进入食物链供应的羔羊的饲养方式（富含淀粉或富含NDF）。
