Reproduction Center of Xuzhou Maternity and Child Health Care Hospital, Xuzhou, Jiangsu 221000, P.R. China.
Mol Med Rep. 2018 Oct;18(4):3850-3858. doi: 10.3892/mmr.2018.9387. Epub 2018 Aug 14.
Endometriosis affects 6‑10% of women of reproductive age. Though a significant amount of research has explored the pathogenesis of endometriosis, little is clear. Elucidating the mechanisms is urgently required for improving the therapeutic efficiency of endometriosis treatment. Long non‑coding RNAs (lncRNAs) have recently acquired extensive attention as regulatory components in a variety of biological processes and diseases. However, the functions of many lncRNAs in endometriosis are poorly understood. Therefore, the exploration of the dysregulated genes in endometriosis, particularly lncRNAs, is of importance. In the present study, datasets for endometriosis, including GSE7305, GSE7846, GSE29981 and E‑MTAB‑694, were downloaded from Gene Expression Omnibus and ArrayExpress. Then, the limma and Affy packages were used to analyze the CEL file. The RankProd method was used to conduct meta‑analysis. Long intergenic non‑protein coding RNA 1279 (LINC01279) was significantly upregulated in the three datasets, and was the most upregulated lncRNA as determined by the RankProd method. Gene set enrichment and Gene Ontology analyses were conducted, which revealed that LINC01279 is likely to function as a cell cycle mediator in endometriosis. Finally, it was identified that LINC01279 is strongly associated with certain previously identified key factors in the development of endometriosis, including cyclin‑dependent kinase 14 and C‑X‑C motif chemokine ligand 12. Thus, it was demonstrated that LINC01279 may be associated with the pathogenesis of endometriosis. This may potentially represent a target in the therapy of endometriosis.
子宫内膜异位症影响 6-10%的育龄妇女。尽管大量研究已经探索了子宫内膜异位症的发病机制，但仍有许多不清楚的地方。阐明这些机制对于提高子宫内膜异位症治疗的疗效是迫切需要的。长链非编码 RNA（lncRNA）最近作为多种生物过程和疾病的调节成分引起了广泛关注。然而，许多 lncRNA 在子宫内膜异位症中的功能仍知之甚少。因此，探索子宫内膜异位症中失调的基因，特别是 lncRNA，是很重要的。在本研究中，从基因表达综合数据库和 ArrayExpress 下载了子宫内膜异位症数据集，包括 GSE7305、GSE7846、GSE29981 和 E-MTAB-694。然后，使用 limma 和 Affy 包分析 CEL 文件。使用 RankProd 方法进行荟萃分析。长基因间非蛋白编码 RNA 1279（LINC01279）在三个数据集均显著上调，并且是 RankProd 方法确定的上调最显著的 lncRNA。进行了基因集富集和基因本体论分析，结果表明 LINC01279可能在子宫内膜异位症中作为细胞周期介质发挥作用。最后，鉴定出 LINC01279与某些先前鉴定的子宫内膜异位症发生的关键因素密切相关，包括细胞周期蛋白依赖性激酶 14 和 C-X-C 基序趋化因子配体 12。因此，证明 LINC01279可能与子宫内膜异位症的发病机制有关。这可能代表子宫内膜异位症治疗的一个潜在靶点。
