Shayanfard Amir Hossein, Salehi Zivar, Mashayekhi Farhad, Zahiri Ziba
Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran.
Department of Obstetrics and Gynaecology, Reproductive Health Research Centre, Alzahra Hospital, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
J Hum Reprod Sci. 2024 Oct-Dec;17(4):246-254. doi: 10.4103/jhrs.jhrs_92_24. Epub 2024 Dec 23.
An increasing number of studies have demonstrated that excessive proliferation and apoptosis play a pivotal role in the development of endometriosis.
The aim of the study was to evaluate the expression of long non-coding RNA (lncRNA) FAS-AS1, FAS, soluble Fas (sFas) and caspase-3 in patients with different stages of endometriosis.
The design of the study was a cross-sectional study.
The relative expression of lncRNA FAS-AS1 and FAs gene was evaluated by the quantitative real-time polymerase chain reaction in 60 ectopic endometrial samples from women with endometriosis in relation to 85 normal endometrial tissues from healthy women, whereas the protein level of sFAs in the peritoneal fluid samples and cleaved caspase-3 in ectopic and normal endometrial tissue samples were determined using the enzyme-linked immunosorbent assay and western blot, respectively. Furthermore, analyses were performed to investigate protein-protein interactions as well as molecular function and cellular location of selected proteins.
The student's -test was used to analyse the difference between the means of the two groups.
The expression of FAS and sFas increased in endometriosis tissues as compared to the control group ( < 0.05). However, lncRNA FAS-AS1 and cleaved caspase-3 decreased in ectopic endometrial tissues compared to normal endometrial tissues and low lncRNA FAS-AS1 expression was correlated with disease stages. In addition, the analysis revealed the importance of FAS/caspase3 in the biological processes involved in the development of endometriosis.
The current study suggests that lncRNA FAS-AS1 may function as an ectopic endometriotic suppressor. Moreover, the results showed that severity of endometriosis is also closely correlated with the expression of lncRNA FAS-AS1 and sFAS.
越来越多的研究表明，过度增殖和凋亡在子宫内膜异位症的发生发展中起关键作用。
本研究旨在评估长链非编码RNA（lncRNA）FAS-AS1、FAS、可溶性Fas（sFas）和半胱天冬酶-3在不同阶段子宫内膜异位症患者中的表达。
本研究设计为横断面研究。
采用定量实时聚合酶链反应评估60例子宫内膜异位症患者异位子宫内膜样本中lncRNA FAS-AS1和FAs基因的相对表达，并与85例健康女性的正常子宫内膜组织进行比较，而腹腔液样本中sFas的蛋白水平以及异位和正常子宫内膜组织样本中裂解的半胱天冬酶-3则分别采用酶联免疫吸附测定和蛋白质印迹法测定。此外，还进行了分析以研究蛋白质-蛋白质相互作用以及所选蛋白质的分子功能和细胞定位。
采用学生t检验分析两组均值之间的差异。
与对照组相比，子宫内膜异位症组织中FAS和sFas的表达增加（P<0.05）。然而，与正常子宫内膜组织相比，异位子宫内膜组织中lncRNA FAS-AS1和裂解的半胱天冬酶-3减少，且lncRNA FAS-AS1低表达与疾病分期相关。此外，分析揭示了FAS/半胱天冬酶3在子宫内膜异位症发生发展相关生物学过程中的重要性。
本研究表明lncRNA FAS-AS1可能作为异位子宫内膜抑制因子发挥作用。此外，结果表明子宫内膜异位症的严重程度也与lncRNA FAS-AS1和sFAS的表达密切相关。
