Department of Gastrointestinal Surgery, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650031, P.R. China.
Department of Anesthesiology, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650031, P.R. China.
Mol Med Rep. 2018 Oct;18(4):3818-3824. doi: 10.3892/mmr.2018.9346. Epub 2018 Aug 3.
The aim of the present study was to investigate the effects of Toll‑like receptor (TLR) 9 and CpG oligodeoxynucleotide (CpG‑ODN)1826 on sodium taurocholate‑induced acute pancreatitis (AP) rats at different time points. Pathological examination indicated that the severity of pancreatic tissue damage following AP increased with time. Additionally, TLR9 protein levels were upregulated after AP, and were higher at 6 h compared with at 3 h. Subsequently, the TLR9 protein levels were downregulated at 12 h, but remained higher than the control group. In rats subjected to AP, tumor necrosis factor (TNF‑α protein expression levels and serum amylase (AMS) in the serum were increased until 12 h. The expression level of TNF‑α protein in the AP 12 h group was higher than that in the AP 3 and 6 h groups. In addition, following CpG‑ODN1826 administration, the morphology of pancreatic tissue appeared worse compared with that in the AP only groups. Furthermore, CpG‑ODN1826 administration induced an increase in TLR9 expression levels compared with the AP alone group at 0, 3, 6 and 12 h. TNF‑α in the CpG + AP 12 h group was upregulated compared with that in the CpG + AP 3 and 6 h groups; however, no change was observed between 3 and 6 h. Thus, these data indicate that CpG‑ODN1826 aggravates sodium taurocholate‑induced pancreas damage in rats.
本研究旨在探讨 Toll 样受体（TLR）9 和 CpG 寡脱氧核苷酸（CpG-ODN）1826 在不同时间点对牛磺胆酸钠诱导的急性胰腺炎（AP）大鼠的影响。病理检查表明，AP 后胰腺组织损伤的严重程度随时间增加而增加。此外，TLR9 蛋白水平在 AP 后上调，6 h 时高于 3 h。随后，TLR9 蛋白水平在 12 h 时下调，但仍高于对照组。在 AP 大鼠中，肿瘤坏死因子（TNF）-α 蛋白表达水平和血清中淀粉酶（AMS）在 12 h 时增加。AP 12 h 组 TNF-α 蛋白表达水平高于 AP 3 和 6 h 组。此外，给予 CpG-ODN1826 后，与 AP 组相比，胰腺组织形态学表现更差。此外，与单独 AP 组相比，CpG-ODN1826 给药在 0、3、6 和 12 h 时诱导 TLR9 表达水平增加。CpG+AP 12 h 组 TNF-α 水平上调，高于 CpG+AP 3 和 6 h 组，但 3 h 和 6 h 组之间无变化。因此，这些数据表明 CpG-ODN1826 加重了牛磺胆酸钠诱导的大鼠胰腺损伤。
