School of Life Science, Nanjing Normal University, Nanjing, Jiangsu 210046, P.R. China.
Department of Surgery, Nanjing Medical University Third Affiliated Hospital, Nanjing, Jiangsu 211166, P.R. China.
Mol Med Rep. 2018 Oct;18(4):3631-3640. doi: 10.3892/mmr.2018.9372. Epub 2018 Aug 9.
Esophageal cancer ranks fourth in cancer‑associated mortality in China and the incidence of esophageal adenocarcinoma has risen dramatically over the past two decades. MicroRNA (miRNA/miR) serves a pivotal role in human cancer cell growth, invasion and migration. MiR‑675‑3p is highly expressed in esophageal squamous cell cancer (ESCC) tissues, and may have an influence on ESCC cell migration and invasion. ESCC tumor tissue samples from 35 patients were profiled. MiR‑675‑3p expression was confirmed by reverse transcription‑quantitative polymerase chain reaction. Manipulation of miR‑675‑3p via knockdown was carried out with subsequent evaluation of effects on cell proliferation, invasion, migration, and use of western blotting and ELISA assays. MiR‑675‑3p was overexpressed in ESCC tissues compared with normal tissues, and had higher expression levels in ESCC cells compared with the healthy esophageal epithelial cell line. The results revealed a predominant upregulation of cell migration and invasion ability. MiR‑675‑3p inhibitor inhibited ESCC cell proliferation, migration and invasion ability. It was also demonstrated that downregulation of miR‑675‑3p decreased the levels of matrix metalloproteinase (MMP) 2 and 9 and increased the level of E‑cadherin. In addition, the effects of miR‑675‑3p inhibitor on ESCC cell lines were eliminated by con‑transfection with miR‑675‑3p inhibitor and miR‑675‑3p mimic. In conclusion, the results indicated that miR‑675‑3p may be involved in the progression of ESCC through regulating ESCC cell migration and invasion capacity via modulating epithelial mesenchymal transition markers (MMP2, MMP 9 and E‑cadherin).
食管癌在中国癌症相关死亡率中排名第四，过去二十年中食管腺癌的发病率急剧上升。微小 RNA（miRNA/miR）在人类癌细胞生长、侵袭和迁移中起关键作用。miR-675-3p 在食管鳞状细胞癌（ESCC）组织中高表达，可能对 ESCC 细胞迁移和侵袭有影响。对 35 例患者的 ESCC 肿瘤组织样本进行了分析。采用逆转录-定量聚合酶链反应（RT-qPCR）验证 miR-675-3p 的表达。通过敲低 miR-675-3p 进行 miR-675-3p 的操作，并随后评估对细胞增殖、侵袭、迁移的影响，以及使用 Western blot 和 ELISA 检测。与正常组织相比，miR-675-3p 在 ESCC 组织中高表达，在 ESCC 细胞中比健康的食管上皮细胞系表达更高。结果表明，细胞迁移和侵袭能力明显上调。miR-675-3p 抑制剂抑制 ESCC 细胞增殖、迁移和侵袭能力。还表明，下调 miR-675-3p 降低了基质金属蛋白酶（MMP）2 和 9 的水平，并增加了 E-钙黏蛋白的水平。此外，miR-675-3p 抑制剂和 miR-675-3p 模拟物的共转染消除了 miR-675-3p 抑制剂对 ESCC 细胞系的影响。总之，结果表明，miR-675-3p 可能通过调节上皮间质转化标志物（MMP2、MMP9 和 E-钙黏蛋白）来调节 ESCC 细胞迁移和侵袭能力，从而参与 ESCC 的进展。
