Chen Dong, Wu Zhong, Luo Lin-Jie, Huang Xiang, Qian Wei-Qiang, Wang Hua, Li Shao-Hua, Liu Jie
Department of Orthopedics, Shanghai Tenth People's Hospital, School of Medicine, Tongji University Shanghai, China.
Int J Clin Exp Pathol. 2015 Nov 1;8(11):14247-51. eCollection 2015.
Cadherins are important adhesion molecules that mediate adhesions and communications between cells. These molecules participate in the formation and maintenance of multicellular organisms including the stem cells. E-cadherin is one of the classic cadherins which is reported to be essential for the survival and self-renewal of embryonic stem cells. Moreover, it could induce cell proliferation inhibitory signaling to regulate cell proliferation. In our study, we over-expressed and silenced E-cadherin in NSCs by lentiviral ways. Transgenic cells were confirmed by both quantitative RT-PCR and western blot. Results of MTT assay showed that over-expression of E-cadherin could enhance the cell activity. Furthermore, we performed Transwell chamber assay to analyze its role in regulation of cell migration. The results showed that the migration percent of over-expression cells was lower than control. Our results indicated that E-caherin would maintain the stemness of NSCs and reduce cells migration.
钙黏蛋白是重要的黏附分子，介导细胞间的黏附与通讯。这些分子参与包括干细胞在内的多细胞生物体的形成与维持。E-钙黏蛋白是经典的钙黏蛋白之一，据报道对胚胎干细胞的存活和自我更新至关重要。此外，它可诱导细胞增殖抑制信号来调节细胞增殖。在我们的研究中，我们通过慢病毒方法在神经干细胞中过表达和沉默E-钙黏蛋白。通过定量RT-PCR和蛋白质印迹法对转基因细胞进行了确认。MTT检测结果表明，E-钙黏蛋白的过表达可增强细胞活性。此外，我们进行了Transwell小室检测以分析其在调节细胞迁移中的作用。结果显示，过表达细胞的迁移百分比低于对照组。我们的结果表明，E-钙黏蛋白可维持神经干细胞的干性并减少细胞迁移。
