Group of Genetics, Breeding and Biochemistry of Brassicas, MisiónBiológica de Galicia, Spanish Council for Scientific Research (CSIC), Pontevedra, Spain.
Group of Genetics, Breeding and Biochemistry of Brassicas, MisiónBiológica de Galicia, Spanish Council for Scientific Research (CSIC), Pontevedra, Spain.
Plant Sci. 2018 Oct;275:28-35. doi: 10.1016/j.plantsci.2018.07.009. Epub 2018 Jul 21.
Coordination of plant circadian rhythms with the external environment provides growth and reproductive advantages to plants as well as enhanced resistance to insects and pathogens. Since glucosinolates (GLSs) play a major role as plant defensive compounds and could affect the palatability and health value of edible crops, the aim of this study was to investigate the species-specific patterns in circadian rhythmicity of these plant phytochemicals. Five different GLS-containing cultivars, from three Brassica crop species were studied. Plants were entrained to light-dark cycles (LD) for five weeks prior to release them into continuous light (LL). GLSs levels were monitored during five consecutive days (two days at LD conditions and three days at LL). The remaining plants were re-entrained to LD cycles (Re-LD plants) and GLS levels were studied as stated before during two consecutive days. Results showed that the period and amplitude of GLSs circadian outputs were cultivar-dependent. In addition, we assessed that the plant endogenous clock can be re-entrained for GLSs accumulation after a period of free-running conditions. Together, these data suggests that Brassica cultivars keep track the time of the day to coordinate their defenses. The demonstration of the cultivar-specific circadian effect on the GLSs levels in plants of different Brassica cultivars have the potential to identify new targets for improving cultivar phytochemicals using temporally informed approaches. In addition, provides an exceptional model to study the complexity of signal integration in plants.
植物的生物钟与外部环境的协调为植物的生长和繁殖提供了优势，同时增强了对昆虫和病原体的抵抗力。由于硫代葡萄糖苷 (GLS) 作为植物防御化合物发挥着重要作用，并且可能影响可食用作物的适口性和营养价值，本研究旨在调查这些植物次生代谢物的生物钟节律的种特异性模式。研究了来自三个芸薹属作物物种的五个不同含有 GLS 的品种。在将植物释放到连续光照 (LL) 之前，它们被驯化到光-暗循环 (LD) 中五周。在连续五天（LD 条件下两天和 LL 条件下三天）监测 GLS 水平。其余植物被重新驯化到 LD 循环（Re-LD 植物），并在连续两天内按照之前的方式研究 GLS 水平。结果表明，GLS 生物钟输出的周期和幅度与品种有关。此外，我们评估了植物内源性时钟在自由运行条件后可以重新为 GLS 积累进行重新调整。总之，这些数据表明芸薹属品种可以根据时间来协调防御。在不同芸薹属品种的植物中，对 GLS 水平的生物钟节律的品种特异性效应的证明有可能通过利用时间相关的方法来识别改善品种次生代谢物的新目标。此外，它为研究植物信号整合的复杂性提供了一个特殊的模型。
