Leibniz Institute of Vegetable and Ornamental Crops, Theodor-Echtermeyer-Weg 1, 14979 Großbeeren, Germany.
Leibniz Institute of Vegetable and Ornamental Crops, Theodor-Echtermeyer-Weg 1, 14979 Großbeeren, Germany.
Food Res Int. 2017 Oct;100(Pt 3):392-403. doi: 10.1016/j.foodres.2017.04.016. Epub 2017 Apr 24.
About 10% of the world's vegetable production is generated from Brassicaceae, wherein Brassica rapa is a dominating species. There is growing evidence that glucosinolates (GLSs), main plant secondary metabolites in Brassicales, play an important role in promoting human health. Natural genetic diversity of B. rapa can be explored for vegetable improvement. We analyzed leaves and tubers of 16 B. rapa turnips for their GLS composition by UHPLC-DAD and the corresponding hydrolysis products by GC-MS. Thirteen GLSs were identified, 8 aliphatic, 4 indolic and one aromatic. 3-Butenyl GLS was prevailing in both plant organs while in tubers 2-hydroxy-3-butenyl GLS and 2-phenylethyl GLS occurred in high amounts. A total of 24 GLS breakdown products were detected in tubers and 16 in leaves. Epithionitriles were the main hydrolysis products in both plant organs with 4,5-epithiopentanenitrile and 3-hydroxy-4,5-epithiopentanenitrile being the main compounds. When comparing leaves and tubers, an accumulation of GLSs and their breakdown products was observed in tubers compared to leaves. Our analysis achieved the comprehensive profiling of all GLS metabolites in a collection of B. rapa turnips, underlining the natural variation not only of intact GLS, but also of their breakdown products.
十字花科植物约占世界蔬菜产量的 10%，其中芸薹属的芸薹种是主要物种。越来越多的证据表明，芥子油苷（GLS）作为芸薹科植物中的主要次生代谢物，在促进人类健康方面发挥着重要作用。可以探索芸薹属植物的自然遗传多样性，以改良蔬菜。我们通过 UHPLC-DAD 分析了 16 个芸薹属萝卜的叶片和块茎中的 GLS 组成，并通过 GC-MS 分析了相应的水解产物。共鉴定出 13 种 GLS，8 种脂肪族，4 种吲哚族和 1 种芳香族。3-丁烯基 GLS 在两种植物器官中均占优势，而在块茎中，2-羟基-3-丁烯基 GLS 和 2-苯乙基 GLS 的含量较高。在块茎中检测到 24 种 GLS 分解产物，在叶片中检测到 16 种。乙硫腈是两种植物器官中的主要水解产物，其中 4,5-乙硫腈和 3-羟基-4,5-乙硫腈是主要化合物。当比较叶片和块茎时，与叶片相比，块茎中 GLS 及其分解产物的积累更多。我们的分析实现了芸薹属萝卜的 GLS 代谢物的全面分析，强调了不仅完整 GLS 存在自然变异，而且其分解产物也存在自然变异。
