Group of Genetics, Breeding and Biochemistry of Brassicas , Misión Biológica de Galicia, Spanish Council for Scientific Research (CSIC) , Pontevedra , Spain.
J Agric Food Chem. 2018 Jun 20;66(24):5984-5991. doi: 10.1021/acs.jafc.8b01732. Epub 2018 Jun 12.
There is increasing evidence that the circadian clock is a significant driver of plant phytochemicals. However, little is known about the clock effect on antioxidant metabolites in edible crops. Thus, the aim of the present investigation was to study whether the antioxidant potential of Brassica cultivars is under circadian regulation and its relationship with polyphenol content. To accomplish that we entrain plants of four Brassica cultivars to light-dark cycles prior to release into continuous light. The antioxidant activity and phenolic content was monitored at four time points of the day during four consecutive days: 2 days under light-dark conditions followed by 2 days under continuous light. Results showed daily oscillation of antioxidant activity. In addition, those variations were related with endogenous circadian rhythms in polyphenolics and exhibit a species-specific pattern. Considered together, we determined that Brassica cultivars have an optimal time during a single day with increased levels of health phytochemicals.
越来越多的证据表明，生物钟是植物次生代谢物的重要驱动因素。然而，关于生物钟对食用作物抗氧化代谢物的影响知之甚少。因此，本研究旨在探讨 Brassica 品种的抗氧化潜力是否受生物钟调节及其与多酚含量的关系。为了实现这一目标，我们在将植物暴露于持续光照之前，将四种 Brassica 品种的植物驯化到光暗循环中。在连续四天的四个时间点监测抗氧化活性和酚类含量：光暗条件下 2 天，然后连续光照 2 天。结果表明抗氧化活性呈每日波动。此外，这些变化与内源性多酚的昼夜节律有关，并表现出特定于物种的模式。综上所述，我们确定 Brassica 品种在一天中的某个最佳时间内具有更高水平的健康植物化学物质。
