Hemeg Hassan A
Department of Clinical Laboratory Sciences, Faculty of Applied Medical Science, Taibah University, Medina 30001, Saudi Arabia.
Saudi J Biol Sci. 2018 Jul;25(5):928-931. doi: 10.1016/j.sjbs.2018.01.016. Epub 2018 Feb 2.
Multidrug-resistant is one of the most important public health concern worldwide that can be transferred through the food of animal origin to human being causing serious infection. The genetic responsibility of such resistant genes (Plasmids, integrons, and transposons) can be easily transmitted from the resistant strain to another. Therefore, the main objectives of the study is the molecular characterization of the resistant isolates recovered from food samples and human isolates collected from outpatient clinics, KSA especially the resistance strains against aminoglycoside resistance genes which are responsible for the resistance against gentamicin and the resistance caused β-lactamases genes. Examination of food samples revealed 120 isolates (22.22%) (30 strains O26: K60, 28 strains O128: K67, 20 strains O111: K58, 18 strains O126: K58, 10 strains O55: K59, 9 strains O86: K61 and 5 strains O157: H7). All the strains were highly resistance to penicillin, amoxicillin-clavulanic and erythromycin with a percentage of 100%, while the resistance to gentamicin, ampicillin, oxytetracycline, chloramphenicol, norfloxacin, trimethoprim, and nalidixic acid were 83%, 75%, 65.3%, 55.8%, 36.5%, 30.7% and 26.9% respectively. On the other hand, 59.6% of tested strains were sensitive to ciprofloxacin. Positive amplification of 896 bp fragments specific for genes were observed by PCR designated for the detection of the aminoglycoside resistance genes. Meanwhile, multiplex PCR designed to detect the ampicillin and amoxicillin-clavulanic acid resistant isolates revealed positive amplification of 516 bp fragments specific for gene with all the resistant strains to ampicillin and amoxicillin-clavulanic acid. Moreover, positive amplification of 392 bp fragments specific for resistant gene were observed with (60.52%) of isolate. While all the tested strains were negative for amplification of .
多重耐药性是全球最重要的公共卫生问题之一，它可通过动物源性食品传播给人类，引发严重感染。此类耐药基因（质粒、整合子和转座子）的遗传特性能够轻易地从耐药菌株传播至另一菌株。因此，本研究的主要目的是对从食品样本以及沙特阿拉伯门诊收集的人类分离株中获得的耐药分离株进行分子特征分析，特别是针对对庆大霉素耐药以及由β-内酰胺酶基因导致耐药的氨基糖苷类耐药基因的耐药菌株。对食品样本的检测发现了120株分离株（22.22%）（30株O26:K60、28株O128:K67、20株O111:K58、18株O126:K58、10株O55:K59、9株O86:K61和5株O157:H7）。所有菌株对青霉素、阿莫西林-克拉维酸和红霉素均具有高度耐药性，耐药率达100%，而对庆大霉素、氨苄西林、土霉素、氯霉素、诺氟沙星、甲氧苄啶和萘啶酸的耐药率分别为83%、75%、65.3%、55.8%、36.5%、30.7%和26.9%。另一方面，59.6%的受试菌株对环丙沙星敏感。通过用于检测氨基糖苷类耐药基因的PCR观察到针对该基因的896 bp片段有阳性扩增。同时，用于检测对氨苄西林和阿莫西林-克拉维酸耐药分离株的多重PCR显示，所有对氨苄西林和阿莫西林-克拉维酸耐药的菌株均出现了针对该基因的516 bp片段的阳性扩增。此外，在60.52%的分离株中观察到针对耐药基因的392 bp片段有阳性扩增。而所有受试菌株对该基因的扩增均为阴性。
