Azab Khaled S M, Abdel-Rahman Mohamed Ali, El-Sheikh Hussien H, Azab Ehab, Gobouri Adil A, Farag Mohamed M S
Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, Egypt.
Al-Azhar Center of Fermentation Biotechnology and Applied Microbiology, Al-Azhar University, Nasr City, Cairo 11884, Egypt.
Antibiotics (Basel). 2021 Mar 2;10(3):247. doi: 10.3390/antibiotics10030247.
The incidence of Extended-spectrum β-lactamase (ESBL)-encoding genes ( and ) among Gram-negative multidrug-resistant pathogens collected from three different countries was investigated. Two hundred and ninety-two clinical isolates were collected from Egypt ( = 90), Saudi Arabia ( = 162), and Sudan ( = 40). Based on the antimicrobial sensitivity against 20 antimicrobial agents from 11 antibiotic classes, the most resistant strains were selected and identified using the Vitek2 system and 16S rRNA gene sequence analysis. A total of 85.6% of the isolates were found to be resistant to more than three antibiotic classes. The ratios of the multidrug-resistant strains for Egypt, Saudi Arabia, and Sudan were 74.4%, 90.1%, and 97.5%, respectively. , , and showed inconstant resistance levels to the different classes of antibiotics. and had the highest levels of resistance against macrolides followed by penicillins and cephalosporin, while was most resistant to penicillins followed by classes that varied among different countries. The isolates were positive for the presence of the and genes. The gene was the predominant gene in all isolates (100%), while was detected in 66.7% of the selected isolates. This work highlights the detection of multidrug-resistant bacteria and resistant genes among different countries. We suggest that the medical authorities urgently implement antimicrobial surveillance plans and infection control policies for early detection and effective prevention of the rapid spread of these pathogens.
对从三个不同国家收集的革兰氏阴性多重耐药病原体中携带超广谱β-内酰胺酶（ESBL）编码基因（blaCTX-M和blaTEM）的情况进行了调查。从埃及（n = 90）、沙特阿拉伯（n = 162）和苏丹（n = 40）收集了292株临床分离株。根据对11类抗生素中的20种抗菌药物的药敏情况，选择最耐药的菌株，并用Vitek2系统和16S rRNA基因序列分析进行鉴定。共发现85.6%的分离株对三类以上抗生素耐药。埃及、沙特阿拉伯和苏丹的多重耐药菌株比例分别为74.4%、90.1%和97.5%。blaCTX-M、blaTEM和blaSHV对不同类别的抗生素表现出不稳定的耐药水平。blaCTX-M和blaTEM对大环内酯类抗生素的耐药水平最高，其次是青霉素类和头孢菌素类，而blaSHV对青霉素类的耐药性最强，其次是在不同国家有所不同的类别。分离株对blaCTX-M和blaTEM基因检测呈阳性。blaCTX-M基因在所有分离株中均占主导地位（100%），而在66.7%的选定分离株中检测到blaTEM。这项工作突出了不同国家多重耐药细菌和耐药基因的检测情况。我们建议医疗当局紧急实施抗菌监测计划和感染控制政策，以便早期发现并有效预防这些病原体的快速传播。
