Department of Restorative Dentistry, Faculty of Dentistry, Eskişehir Osmangazi University, Eskişehir, Turkey.
Department of Endodontics, Faculty of Dentistry, Eskişehir Osmangazi University, Eskişehir, Turkey.
PLoS One. 2018 Aug 15;13(8):e0202081. doi: 10.1371/journal.pone.0202081. eCollection 2018.
Mixing sodium hypochlorite (NaOCl) with chlorhexidine (CHX) forms a brown precipitate. QMix-2in1 (QMix) was introduced as a final irrigant. Manufacturer recommends interim flushing with saline between the application of NaOCl and QMix to prevent formation of precipitation. This study assessed whether para-chloroaniline (PCA) is formed after mixing NaOCl with QMix.
Commercially available, 5.25% NaOCl solution, 2% CHX, QMix, 15% ethylenediaminetetraacetic acid (EDTA) and 98% PCA in powder form were used. Groups were prepared at room temperature. Group 1, 98% PCA in powder form; Group 2, 2% chlorhexidine (CHX); Group 3, QMix; Group 4, 5.25% sodium hypochlorite (NaOCl) mixed with QMix; Group 5, 5.25% NaOCl mixed with CHX; Group 6, 15% EDTA mixed with CHX. The precipitates were extracted and analysed with Proton Nuclear Magnetic Resonance (1H-NMR) and Infrared (IR) Spectroscopy, using PCA as an internal standard.
No PCA was found in any of the irrigant-mixture groups tested.
This study used the interpretation of spectral results for the amino signals of precipitate formed after mixing QMix with 5.25% NaOCl using different nondestructive analysis methods, with PCA as an internal standard (control). We conclude that mixing QMix or 2%CHX with 5.25% NaOCl does not yield free PCA.
次氯酸钠（NaOCl）与洗必泰（CHX）混合会形成棕色沉淀。QMix-2in1（QMix）作为最终冲洗液被引入。制造商建议在 NaOCl 和 QMix 之间应用之间用生理盐水进行中间冲洗，以防止沉淀形成。本研究评估了将 QMix 与 NaOCl 混合后是否形成对氯苯胺（PCA）。
使用市售的 5.25% NaOCl 溶液、2% CHX、QMix、15%乙二胺四乙酸（EDTA）和粉末形式的 98% PCA。在室温下制备组。第 1 组：粉末形式的 98% PCA；第 2 组：2% CHX；第 3 组：QMix；第 4 组：与 QMix 混合的 5.25%次氯酸钠（NaOCl）；第 5 组：与 CHX 混合的 5.25% NaOCl；第 6 组：与 CHX 混合的 15% EDTA。使用 PCA 作为内标，提取沉淀并进行质子核磁共振（1H-NMR）和红外（IR）光谱分析。
在所测试的冲洗液混合组中均未发现 PCA。
本研究使用不同的非破坏性分析方法，使用 PCA 作为内标（对照），对 QMix 与 5.25% NaOCl 混合后形成的沉淀的氨基信号的光谱结果进行解释。我们得出结论，混合 QMix 或 2%CHX 与 5.25% NaOCl 不会产生游离 PCA。
