针对不同分化阶段B淋巴细胞制备的异种抗血清。I. 血清的制备及对不同器官淋巴细胞的细胞毒性
Heteroantisera prepared against B lymphocytes at different stages of differentiation. I. Preparation of sera and cytotoxicity to lymphoid cells from different organs.
作者信息
Gorczynski R M
出版信息
Immunology. 1977 May;32(5):709-15.
Abstract
A routine method is described whereby anti-mouse B-cell antisera can be raised in rabbits. As judged by trypan blue dye exclusion these sera have negligible cytotoxicity to murine T lymphocytes but seem to detect differences in non-T lymphocytes in different organs. By sedimentation analysis some of the sera may detect (in addition to mature B cells) earlier precursors of mature B lymphocytes in bone marrow and spleen.
摘要
本文描述了一种常规方法，通过该方法可在兔体内产生抗小鼠B细胞抗血清。通过台盼蓝拒染法判断，这些血清对小鼠T淋巴细胞的细胞毒性可忽略不计，但似乎能检测出不同器官中非T淋巴细胞的差异。通过沉降分析，部分血清（除成熟B细胞外）可能还能检测到骨髓和脾脏中成熟B淋巴细胞的早期前体细胞。
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引用本文的文献
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Effect of gold sodium thiomalate on murine lymphocyte functions.硫代苹果酸钠金对小鼠淋巴细胞功能的影响。
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本文引用的文献
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Separation of cells by velocity sedimentation.通过速度沉降法分离细胞。
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