Yutoku M, Grossberg A L, Pressman D
J Immunol. 1975 Jul;115(1):69-74.
A new cell-surface antigen of mouse lymphocytes, which was found earlier on normal and malignant plasma cells and about half the normal thymus cell population, has been shown to be on mature B cells and on the least immunocompetent subpopulation of cells of the thymus. The antigen was first detected by the cytotoxic effect on relevant cells of an in vivo purified rabbit antiserum raised against cells of the mouse IgM-producing plasma cell tumor MOPC-104E. We have now used a cell-transfer method to examine the effect of this antiserum (RantiM104E) on immunocompetent lymphocytes of spleen, including separated splenic B and T cells and thymus and bone marrow cells. We have found the antigen on the immunocompetent B lymphocytes of the spleen, but not on any other of the immunocompetent cells examined. The method involves determining the effect of the antiserum on the development of plaque-forming cells in the spleens of irradiated mice that have received lymphocytes from immune or non-immune donors by treating the lymphocyyes with RantiM104E antiserum before transfer. Plaque-forming cells are suppressed when antiserum-treated spleen cells are transferred to the irradiated mice.This suppression was found to be due to action on only the splenic B cells. The helper activity of splenic T cells was not reduced significantly by antiserum treatment. Neither was the helper activity of thymus cells similarly treated reduced by the antiserum. We found, in fact, that the subpopulation of the thymus cells which is resistant to the antiserum was more immunocompetent than the total thymus cell population. In addition, the hydrocortisone-resistant thymus cells were also found to be resistant to the cytotoxic action of the antiserum. The antiserum had no effect on bone marrow cells in the cell-transfer procedure. The antigen involved, which we are designating "Th-B" appears to be a B cell line marker which appears relatively early during the differentiation of mouse B cells from precursor cells and is lost during maturation of T cells.
一种新的小鼠淋巴细胞表面抗原，早前在正常和恶性浆细胞以及约一半的正常胸腺细胞群体中被发现，现已证明它存在于成熟B细胞和胸腺中免疫活性最低的细胞亚群上。该抗原最初是通过对体内纯化的兔抗血清对相关细胞的细胞毒性作用检测到的，该抗血清是针对产生小鼠IgM的浆细胞瘤MOPC - 104E的细胞产生的。我们现在使用细胞转移方法来检测这种抗血清（抗RantiM104E）对脾脏免疫活性淋巴细胞的影响，包括分离的脾脏B细胞和T细胞以及胸腺和骨髓细胞。我们在脾脏的免疫活性B淋巴细胞上发现了该抗原，但在所检测的任何其他免疫活性细胞上均未发现。该方法包括通过在转移前用抗RantiM104E抗血清处理淋巴细胞，来确定抗血清对接受来自免疫或非免疫供体淋巴细胞的受辐照小鼠脾脏中形成空斑细胞发育的影响。当用抗血清处理的脾细胞转移到受辐照小鼠体内时，形成空斑细胞受到抑制。发现这种抑制仅归因于对脾脏B细胞的作用。抗血清处理并未显著降低脾脏T细胞的辅助活性。同样处理的胸腺细胞的辅助活性也未被抗血清降低。事实上，我们发现对该抗血清有抗性的胸腺细胞亚群比整个胸腺细胞群体的免疫活性更强。此外，还发现对氢化可的松有抗性的胸腺细胞对该抗血清的细胞毒性作用也有抗性。在细胞转移过程中，该抗血清对骨髓细胞没有影响。所涉及的这种抗原，我们将其命名为“Th - B”，似乎是一种B细胞系标记物，它在小鼠B细胞从前体细胞分化过程中相对较早出现，并在T细胞成熟过程中消失。
