Yang Juan, Gong Zhuandi, Shen Xiaoyun, Bai Shengju, Bai Xiaoqiang, Wei Suocheng
College of Life Science and Engineering, Northwest Minzu University, Lanzhou, Gansu, P. R. China.
Hospital of Medicine College, Northwest Minzu University, Lanzhou, Gansu, P. R. China.
Anim Biotechnol. 2021 Feb;32(1):84-91. doi: 10.1080/10495398.2019.1656083. Epub 2019 Aug 28.
The present study aimed to explore FSH receptor binding inhibitor (FRBI) effects on the levels of c-Myc, K-Ras and VEGF related to ovarian cancer, to evaluate the mRNA and protein levels of FSHR in the cumulus-oocyte complex (COCs). COCs were cultured for 24 h in the maturation (IVM) media replenished with 0, 10, 20, 30 and 40 μg/mL FRBI. Contents of c-Myc, K-Ras, VEGF, cAMP and IP3 in IVM media were detected with ELISA kits, respectively. The results indicated that the levels of FSHR protein and mRNA were determined with Western blotting. C-Myc contents of four FRBI + FSH-treated groups (COM groups) were reduced after IVM of COCs. C-Myc concentrations of COM-3 group was lower than the FSH group ( < .05). K-Ras and IP3 contents of COM-4 were decreased as compared to FSH group ( < .05). Expression levels of FSHR mRNAs and proteins in COM-4 group were smaller than that of FSH group. This study revealed that FRBI treatment could decrease c-Myc and K-Ras levels in the IVM medium fluids, and depress the FSHR levels of COCs. Expression levels of FSHR mRNAs and proteins of COM-4 group were significantly decreased. FRBI exerted its action via the signal pathway of IP3 and cAMP.
本研究旨在探讨促卵泡激素受体结合抑制剂（FRBI）对卵巢癌相关的c-Myc、K-Ras和血管内皮生长因子（VEGF）水平的影响，评估卵丘-卵母细胞复合体（COCs）中促卵泡激素受体（FSHR）的mRNA和蛋白质水平。将COCs在补充有0、10、20、30和40μg/mL FRBI的成熟（IVM）培养基中培养24小时。分别用ELISA试剂盒检测IVM培养基中c-Myc、K-Ras、VEGF、环磷酸腺苷（cAMP）和三磷酸肌醇（IP3）的含量。结果表明，用蛋白质印迹法测定FSHR蛋白和mRNA水平。COCs体外成熟（IVM）后，四个FRBI+FSH处理组（COM组）的c-Myc含量降低。COM-3组的c-Myc浓度低于FSH组（P<0.05）。与FSH组相比，COM-4组的K-Ras和IP3含量降低（P<0.05）。COM-4组FSHR mRNA和蛋白的表达水平低于FSH组。本研究表明，FRBI处理可降低IVM培养基液中的c-Myc和K-Ras水平，并降低COCs的FSHR水平。COM-4组FSHR mRNA和蛋白的表达水平显著降低。FRBI通过IP3和cAMP信号通路发挥作用。
