Zyablitsin A V, Dmitriev A A, Krasnov G S, Bolsheva N L, Rozhmina T A, Muravenko O V, Fedorova M S, Snezhkina A V, Kudryavtseva A V, Melnikova N V
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia.
All-Russian Research Institute for Flax, Torzhok, 172002 Russia.
Mol Biol (Mosk). 2018 Jul-Aug;52(4):595-600. doi: 10.1134/S0026898418040195.
Understanding the molecular mechanisms of plant response to unfavorable conditions is necessary for the effective selection of tolerant genotypes. Earlier, using high-throughput transcriptome sequencing of flax plants after exposure to aluminum ions (Al^(3+)) and high soil acidity, we detected stress-induced alteration in the expression of several genes, including CAX3, which encodes Ca^(2+)/H^(+)-exchanger involved in calcium ion transport. Here we describe CAX3 mRNA levels in flax cultivars either tolerant (Hermes and TMP1919) or sensitive (Lira and Orshanskiy) to Al^(3+). Stress-induced increased expression of CAX3 was detected only in aluminum-tolerant flax cultivars. The product of CAX3 gene may participate in flax response to high soil acidity and high Al^(3+) concentration through Ca^(2+)-mediated intracellular regulation.
了解植物对不利条件的分子响应机制对于有效选择耐性基因型至关重要。此前，通过对暴露于铝离子（Al³⁺）和高土壤酸度后的亚麻植株进行高通量转录组测序，我们检测到了几个基因表达的应激诱导变化，包括CAX3，它编码参与钙离子运输的Ca²⁺/H⁺交换蛋白。在此，我们描述了对Al³⁺耐性（Hermes和TMP1919）或敏感（Lira和Orshanskiy）的亚麻品种中CAX3 mRNA水平。仅在耐铝亚麻品种中检测到应激诱导的CAX3表达增加。CAX3基因的产物可能通过Ca²⁺介导的细胞内调节参与亚麻对高土壤酸度和高Al³⁺浓度的响应。
