Immunology Department, Habib Bourguiba Hospital, University of Sfax, Sfax, Tunisia.
Dermatology Department, Hedi Chaker Hospital, University of Sfax, Sfax, Tunisia.
Mediators Inflamm. 2018 Jul 12;2018:8206983. doi: 10.1155/2018/8206983. eCollection 2018.
Pemphigus foliaceus (PF) is a rare autoimmune skin disease caused by anti-Dsg1 pathogenic autoantibodies. It is considered as a Th2-mediated disease. Likewise, Th17 cells were recently described in the pathogenesis of the disease but their role is still unclear. We aimed to unravel the eventual implication of the IL23/Th17 pathway in the development of PF. A case-control study was conducted on 115 PF patients and 201 healthy controls using PCR-RFLP and AS-PCR methods. SNPs in , , , , , , and genes were genotyped. mRNA expression of and was evaluated using Q-PCR. The frequency of circulating Th17 cells was analyzed by flow cytometry. Genetic associations between >rs11209026, >rs3748067, >rs763780, and >rs1800629 and the susceptibility to PF were reported. Moreover, we revealed a significant increased frequency of circulating CD4IL17 cells as well as higher mRNA levels of RORt and IL23R in PBMCs of patients. However, no significant increase of RORt and IL23R mRNA expression was observed in lesional skin biopsies. In spite of the little size of specimens, our results provide converging arguments for the contribution of the IL23/Th17 pathway in the pathogenesis of PF.
落叶型天疱疮（PF）是一种罕见的自身免疫性皮肤病，由抗 Dsg1 致病性自身抗体引起。它被认为是一种 Th2 介导的疾病。同样，Th17 细胞最近也被描述在疾病的发病机制中，但它们的作用仍不清楚。我们旨在揭示 IL23/Th17 通路在 PF 发展中的潜在作用。采用 PCR-RFLP 和 AS-PCR 方法对 115 例 PF 患者和 201 例健康对照进行了病例对照研究。对 、 、 、 、 、 基因中的 SNPs 进行了基因分型。采用 Q-PCR 法评估 、 的 mRNA 表达。通过流式细胞术分析循环 Th17 细胞的频率。报告了 >rs11209026、>rs3748067、>rs763780 和 >rs1800629 与 PF 易感性之间的遗传关联。此外，我们发现患者 PBMC 中循环 CD4IL17 细胞的频率显著增加，RORt 和 IL23R 的 mRNA 水平也升高。然而，在皮损皮肤活检中未观察到 RORt 和 IL23R mRNA 表达的显著增加。尽管标本数量较少，但我们的结果提供了支持 IL23/Th17 通路在 PF 发病机制中作用的有力证据。
