Yamashina S, Katsumata O, Wada I, Kato K
Histochemistry. 1986;84(3):231-6. doi: 10.1007/BF00495787.
The localization of 5'-nucleotidase in rat parotid and submandibular glands was investigated at the electron microscope level by an immunohistochemical technique using a highly specific antibody, and the results were compared with those obtained using the newley developed cerium method for enzyme histochemistry. Both methods demonstrated that 5'-nucleotidase is located on the external surface of the luminal plasma membranes of acinar cells as well as on intercalated and striated ductal cells. In the basolateral membranes of these cells, the portions adjacent to myoepithelial cells exhibited intense reaction products, but the other areas of plasma membranes contained only trace amounts of the reaction products. Both cerium-based enzyme histochemistry and immunohistochemistry showed that myoepithelial cells retain the enzyme on their plasma membranes. Neither method produced reaction products in the intracytoplasmic structure of constitutive cells of the salivary glands. We discuss the usefulness of the cerium-ion method for the demonstration of 5'-nucleotidase activity and compare it with the traditional lead-ion method.
利用高特异性抗体，通过免疫组织化学技术在电子显微镜水平研究了5'-核苷酸酶在大鼠腮腺和颌下腺中的定位，并将结果与使用新开发的铈法进行酶组织化学所得结果进行比较。两种方法均表明，5'-核苷酸酶位于腺泡细胞腔面膜的外表面以及闰管和纹状管细胞上。在这些细胞的基底外侧膜中，与肌上皮细胞相邻的部分呈现强烈的反应产物，但质膜的其他区域仅含有微量反应产物。基于铈的酶组织化学和免疫组织化学均显示，肌上皮细胞质膜上保留有该酶。两种方法均未在唾液腺组成细胞的胞质结构中产生反应产物。我们讨论了铈离子法在显示5'-核苷酸酶活性方面的实用性，并将其与传统的铅离子法进行比较。
