Prescott S M, Zimmerman G A, Seeger A R
Biochem Biophys Res Commun. 1984 Jul 31;122(2):535-41. doi: 10.1016/s0006-291x(84)80066-2.
Leukotriene B4 is the major biologically-active metabolite of arachidonate in stimulated neutrophils and previously has been shown to be a potent stimulus for neutrophil chemotaxis and aggregation. It is a less potent agonist for the secretion of granular contents from neutrophils. In the present study we have shown that leukotriene B4 does not activate all of the neutrophil responses seen with chemotactic peptides or the calcium ionophore A23187. This conclusion is based on the failure of exogenous leukotriene B4 to induce neutrophils to: (1) synthesize 5-hydroxyeicosatetraenoic acid or leukotriene B4 from endogenous arachidonate, (2) release arachidonate from membrane lipids, or (3) synthesize platelet-activating factor.
白三烯B4是刺激的中性粒细胞中花生四烯酸的主要生物活性代谢产物，此前已被证明是中性粒细胞趋化性和聚集的有效刺激物。它是中性粒细胞颗粒内容物分泌的较弱激动剂。在本研究中，我们已表明白三烯B4不会激活趋化肽或钙离子载体A23187所引发的所有中性粒细胞反应。这一结论基于外源性白三烯B4未能诱导中性粒细胞：(1) 从内源性花生四烯酸合成5-羟基二十碳四烯酸或白三烯B4，(2) 从膜脂中释放花生四烯酸，或(3) 合成血小板活化因子。
