Purves F C, Katan M, Stevely W S, Leader D P
J Gen Virol. 1986 Jun;67 ( Pt 6):1049-57. doi: 10.1099/0022-1317-67-6-1049.
The appearance of a recently described protein kinase activity (virus-induced protein kinase, ViPK) has been studied during infection of hamster fibroblasts with pseudorabies virus or with herpes simplex virus type 1 (HSV-1). An enzyme activity with comparable catalytic properties was induced in both cases, and had broadly similar kinetics of appearance to that of the viral DNA polymerase. The amount of active ViPK detected depended on the multiplicity of infection, and no ViPK was induced after the viruses had been subjected to irradiation with u.v. light. When cells were infected with the tsK mutant of HSV-1, ViPK was induced at the permissive but not at the restrictive temperature. The ViPK preparations obtained from cells infected with each virus differed in chromatographic properties on anion-exchange and gel-permeation resins. These results indicate that expression of the viral genome is required for induction of ViPK. They suggest that the enzyme may be encoded by the viral genome, but do not provide proof of this.
在仓鼠成纤维细胞被伪狂犬病病毒或1型单纯疱疹病毒（HSV-1）感染期间，对最近描述的一种蛋白激酶活性（病毒诱导的蛋白激酶，ViPK）的出现情况进行了研究。在这两种情况下均诱导出了具有类似催化特性的酶活性，其出现的动力学与病毒DNA聚合酶的动力学大致相似。检测到的活性ViPK的量取决于感染复数，在用紫外线照射病毒后未诱导出ViPK。当细胞被HSV-1的tsK突变体感染时，在允许温度而非限制温度下诱导出ViPK。从感染每种病毒的细胞中获得的ViPK制剂在阴离子交换树脂和凝胶渗透树脂上的色谱特性有所不同。这些结果表明，ViPK的诱导需要病毒基因组的表达。它们提示该酶可能由病毒基因组编码，但未提供这方面的证据。
