Galil A, Antverg R, Katzir G, Zentner B, Margalith M, Friedman M G, Sarov B, Sarov I
J Med Virol. 1986 Apr;18(4):317-26. doi: 10.1002/jmv.1890180404.
An outbreak of acute gastroenteritis in a kibbutz in southern Israel, characterized by diarrhea, fever, vomiting, and abdominal pain, involved 32 kibbutz members of all ages. Nineteen percent of the children and 3.5% of the adults were ill. Transmission of the illness occurred in direct proportion to the degree of close contact, involving first infants, then mothers and nursery staff, and only later youngsters, adolescents, and fathers. Stool samples obtained from 32 kibbutz members with clinical illness and from 44 asymptomatic close contacts were examined for the presence of rotavirus antigen. Fifty-six percent of symptomatic members were positive for rotavirus antigen as compared with 4.5% of asymptomatic close contacts. Positivity of stool samples correlated inversely with the number of days elapsed after onset of illness until the sample was obtained. Serologic studies carried out on acute and convalescent sera of symptomatic and asymptomatic subjects further supported a rotavirus etiology for the outbreak. RNA profiles of stool sample extracts obtained by polyacrylamide gel electrophoresis and silver staining indicate that one electropherotype may have been responsible for the outbreak.
以色列南部一个基布兹爆发了急性肠胃炎，症状为腹泻、发烧、呕吐和腹痛，涉及该基布兹所有年龄段的32名成员。19%的儿童和3.5%的成年人患病。疾病传播与密切接触程度成正比，首先累及婴儿，然后是母亲和托儿所工作人员，较晚才累及儿童、青少年和父亲。对32名有临床症状的基布兹成员以及44名无症状密切接触者的粪便样本进行了轮状病毒抗原检测。56%有症状的成员轮状病毒抗原呈阳性，而无症状密切接触者中这一比例为4.5%。粪便样本的阳性率与发病后至采集样本之间的天数呈负相关。对有症状和无症状受试者的急性期和恢复期血清进行的血清学研究进一步支持了此次疫情由轮状病毒引起的病因。通过聚丙烯酰胺凝胶电泳和银染获得的粪便样本提取物的RNA图谱表明，一种电泳型可能是此次疫情的病因。
