Li Huan-ping, Xin Qi, Lu Jun, Yuan Miao-miao, Pervaiz Nabeel, Jing Tao
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2016 Feb;34(1):18-22.
To study the effect of Echinococcus multilocularis infection on the activities of drug-metabolizing enzymes in gerbil liver.
Ten gerbils （Meriones unguiculatus） were randomly divided into 2 groups, the experimental group in which each animal was intraperitoneally injected with 300 μl E. multilocularis cyst homogenates （containing about 600 protoscoleces）, and the control group in which each animal received 300 μl saline（i.p.）. Five months after infection, the animals were sacrificed by cervical dislocation, and livers were collected. The liver microsomal and cytosolic fractions were obtained by differential centrifugation. Protein concentration was measured by the BCA method. CYP450 and Cyt b5 contents in the microsomal fraction were measured with differential spectroscopy. Activities of 7-ethoxyresorufin（EROD） and 7-methoxyresorufin（MROD） were measured by fluorescence spectrometry. The activities of NADPH-cytochrome C reductase（NCR）, glutathione-S-transferase （GST）, and flavine monooxygenases （FMO） were measured by UV-visible spectrophotometry.
The protein content of cytosolic fractions and liver microsomes in experimental group was （11.089±1.277） and （3.212±0.924） mg/ml， those in control group was （12.459±1.625） and （3.894±0.395） mg/ml， respectively. The contents of CYP450 and Cyt b5 in the experimental group [（0.508±0.142）, （0.515±0.077） nmol/mg protein, respectively] were both significantly lower than those in the control [（0.647±0.090）, （0.596±0.051） nmol/mg protein]（P<0.05）. The GST activity decreased significantly in the experimental group ［（1.766±0.339）×103 nmol/（mg·min）］ compared with the control ［（2.001±0.160）×103 nmol/（mg·min）］（P<0.05）. But the FMO and NCR activities increased significantly in the experimental group ［（1.142±0.327） nmol/（mg·min） and （0.602±0.162）×103 nmol/（mg·min）, respectively］ compared with the control ［（0.882±0.150） nmol/（mg·min） and （0.442±0.082）×103 nmol/（mg·min）］（P<0.05）. However, there were no significant differences in EROD and MROD activities between the two groups (P>0.05).
The activities of FMO and NCR markedly increase, while that of GST significantly decreases in the gerbil liver after E. multilocularis infection.
研究多房棘球绦虫感染对沙鼠肝脏药物代谢酶活性的影响。
将10只长爪沙鼠随机分为2组，实验组每只动物腹腔注射300 μl多房棘球绦虫囊肿匀浆（含约600个原头节），对照组每只动物腹腔注射300 μl生理盐水。感染5个月后，通过颈椎脱臼法处死动物，采集肝脏。采用差速离心法获得肝脏微粒体和胞质部分。用BCA法测定蛋白质浓度。用差示光谱法测定微粒体部分的CYP450和细胞色素b5含量。用荧光光谱法测定7-乙氧基试卤灵（EROD）和7-甲氧基试卤灵（MROD）的活性。用紫外可见分光光度法测定NADPH-细胞色素C还原酶（NCR）、谷胱甘肽-S-转移酶（GST）和黄素单加氧酶（FMO）的活性。
实验组胞质部分和肝脏微粒体的蛋白质含量分别为（11.089±1.277）和（3.212±0.924）mg/ml，对照组分别为（12.459±1.625）和（3.894±0.395）mg/ml。实验组中CYP450和细胞色素b5的含量[分别为（0.508±0.142）、（0.515±0.077）nmol/mg蛋白质]均显著低于对照组[（0.647±0.090）、（0.596±0.051）nmol/mg蛋白质]（P<0.05）。与对照组[（2.001±0.160）×103 nmol/（mg·min）]相比，实验组的GST活性显著降低[（1.766±0.339）×103 nmol/（mg·min）]（P<0.05）。但与对照组[（0.882±0.150）nmol/（mg·min）和（0.442±0.082）×103 nmol/（mg·min）]相比，实验组的FMO和NCR活性显著增加[分别为（1.142±0.327）nmol/（mg·min）和（0.602±0.162）×103 nmol/（mg·min）]（P<0.05）。然而，两组之间的EROD和MROD活性没有显著差异（P>0.05）。
多房棘球绦虫感染后，沙鼠肝脏中FMO和NCR的活性显著增加，而GST的活性显著降低。
