Cao Sheng-kui, Pan Wei, Liu Hua, Cao Jian-ping, Shen Yu-juan
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2016 Feb;34(1):27-31.
To investigate the alteration of expression and activity of arginase from monocytic-type myeloid-derived suppressor cells（M-MDSC） in BALB/c mice infected with Echinococcus granulosus.
Twelve BALB/c female mice were randomly divided into control and infected groups. The mice were injected intraperitoneally with 2 000 live protoscoleces or an equivalent volume of normal saline. After 120 days, peripheral blood was collected through venae orbitaeta, and mice were sacrificed for pathological examination. The spleen was collected under aseptic conditions and single-cell suspension was prepared for M-MDSC isolation using the magnetic bead separation technology. Total RNA was extracted from M-MDSC, cDNA was generated, and genes with differential expression without and with infection were screened using the chip hybridization method. The resulting genes were further validated using real-time PCR. The activity of arginase from peripheral blood was also measured.
Single cyst was formed within the abdomen and internal organs 120 days after infection. Chip hybridization and real-time PCR showed that the relative expression of arginase from M-MDSC in the infected group （7.92±0.85 and 11.97±5.39, respectively） was significantly higher than that in the control group （1.65±0.19 and 1.00±0.57, respectively） （P<0.05）. The activity of arginase was also significantly higher in the infected group ［（3.83±0.44）U/L］ than in the control [(1.57±0.57）U/L].
The expression and activity of arginase from mouse M-MDSC both increase significantly after infection with Echinococcus granulosus.
研究感染细粒棘球绦虫的BALB/c小鼠单核细胞型髓源性抑制细胞（M-MDSC）中精氨酸酶表达及活性的变化。
将12只雌性BALB/c小鼠随机分为对照组和感染组。给小鼠腹腔注射2000个活原头节或等量生理盐水。120天后，通过眶静脉采集外周血，并处死小鼠进行病理检查。无菌条件下采集脾脏，制备单细胞悬液，采用磁珠分离技术分离M-MDSC。提取M-MDSC中的总RNA，逆转录生成cDNA，采用芯片杂交法筛选感染前后差异表达的基因。用实时荧光定量PCR进一步验证所得基因。同时检测外周血中精氨酸酶的活性。
感染120天后，小鼠腹腔及内脏形成单个囊肿。芯片杂交和实时荧光定量PCR结果显示，感染组M-MDSC中精氨酸酶的相对表达量（分别为7.92±0.85和11.97±5.39）显著高于对照组（分别为1.65±0.19和1.00±0.57）（P<0.05）。感染组精氨酸酶活性［（3.83±0.44）U/L］也显著高于对照组［（1.57±0.57）U/L］。
感染细粒棘球绦虫后，小鼠M-MDSC中精氨酸酶的表达及活性均显著增加。
