Haertle T, Orgel L E
J Mol Biol. 1986 Mar 5;188(1):77-80. doi: 10.1016/0022-2836(86)90482-1.
When the deoxynucleotide template d(C7-G-C7) is incubated with the activated nucleotides 2-MeImpG and 2-MeImpC, a series of oligomers of G up to the sevenmer and a series of copolymers of composition GnC with n = 3 to 13 are formed. Oligomers GnC with n greater than 7 are completely degraded by pancreatic ribonuclease, establishing that they contain a 3' to 5' internucleotide bond between 5'-C and 3'-G within a sequence of the form (pG)ipC(pG)j. As expected, (pG)7-Cp and (pG)6-Cp are major hydrolysis products. Detailed analysis of the product distribution shows that a substantial fraction of the oligomeric products are of the type (pG)ipC(pG)j with i less than 7. This shows that product synthesis does not necessarily begin at the 3' terminus of the template. The significance of this finding in terms of the origin of molecular replication is discussed.
当脱氧核苷酸模板d(C7 - G - C7)与活化的核苷酸2 - MeImpG和2 - MeImpC一起孵育时，会形成一系列直至七聚体的G寡聚物以及一系列组成GnC（n = 3至13）的共聚物。n大于7的寡聚物GnC会被胰核糖核酸酶完全降解，这表明它们在形式为(pG)ipC(pG)j的序列中5'-C和3'-G之间含有3'至5'的核苷酸间键。正如预期的那样，(pG)7 - Cp和(pG)6 - Cp是主要的水解产物。对产物分布的详细分析表明，相当一部分寡聚产物是i小于7的(pG)ipC(pG)j类型。这表明产物合成不一定从模板的3'末端开始。本文讨论了这一发现对于分子复制起源的意义。
