Inoue T, Joyce G F, Grzeskowiak K, Orgel L E, Brown J M, Reese C B
J Mol Biol. 1984 Sep 25;178(3):669-76. doi: 10.1016/0022-2836(84)90244-4.
The pentanucleotide CpCpGpCpC facilitates the synthesis of oligomers containing G and C from a mixture of the two activated mononucleotides (guanosine 5'-phosphor)-2-methylimidazolide and (cytidine 5'-phosphor)-2-methylimidazolide. The major pentameric product of the template-directed reaction is all 3' to 5'-linked and has the sequence pGpGpCpGpG, which is complementary to that of the template. It can be obtained in a yield of up to 17%, based on the input of the template. The 3' to 5' isomer of GpG is elongated on the template to give GpGpC, GpGpCpG and GpGpCpGpG, while the 2' to 5' isomer does not initiate the synthesis of detectable amounts of longer oligomers.
五核苷酸CpCpGpCpC可促进从两种活化单核苷酸（鸟苷5'-磷酸）-2-甲基咪唑酯和（胞苷5'-磷酸）-2-甲基咪唑酯的混合物中合成含G和C的寡聚物。模板导向反应的主要五聚体产物全部为3'至5'-连接，其序列为pGpGpCpGpG，与模板序列互补。基于模板的输入量，其产率可达17%。GpG的3'至5'异构体在模板上延伸生成GpGpC、GpGpCpG和GpGpCpGpG，而2'至5'异构体则不会引发可检测量的更长寡聚物的合成。
