Joyce G F, Orgel L E
J Mol Biol. 1986 Apr 5;188(3):433-41. doi: 10.1016/0022-2836(86)90166-x.
Poly(C, G) random copolymer templates direct the oligomerization of 2-Me-ImpG and 2-MeImpC, resulting in the production of a variety of oligo(G, C)s. The efficiency of monomer incorporation into newly synthesized oligomers is greater for 2-MeImpG than for 2-MeImpC, and decreases for both monomers as the guanine content of the template increases. The relatively low efficiency of oligomerization on guanine-rich templates is largely a consequence of intra- and intermolecular template self-structure. The problem of template self-structure is clearly a major obstacle to the development of a system of self-replicating polynucleotides. The distribution of oligomeric products can be characterized in detail using high-pressure liquid chromatography on an RPC-5 column. Oligomers are separated on the basis of chain length, base composition and phosphodiester-linkage isomerism. Oligomers up to about the 12-mer, with base composition Gn, Gn-1C and Gn-2C2, have been identified. The 3' to 5' regiospecificity of the products is high, particularly for oligomers with base composition Gn.
聚（C，G）随机共聚物模板指导2-甲基异鸟苷（2-Me-ImpG）和2-甲基异胞苷（2-MeImpC）的寡聚化，从而产生多种寡聚（G，C）。2-甲基异鸟苷掺入新合成寡聚物中的效率高于2-甲基异胞苷，并且随着模板鸟嘌呤含量的增加，两种单体的掺入效率均降低。富含鸟嘌呤的模板上寡聚化效率相对较低主要是分子内和分子间模板自身结构的结果。模板自身结构问题显然是自我复制多核苷酸系统发展的主要障碍。使用RPC-5柱上的高压液相色谱法可以详细表征寡聚产物的分布。寡聚物根据链长、碱基组成和磷酸二酯键异构性进行分离。已鉴定出碱基组成为Gn、Gn-1C和Gn-2C2的约12聚体以下的寡聚物。产物的3'到5'区域特异性很高，特别是对于碱基组成为Gn的寡聚物。
