Key Laboratory of Geographic Information Science (Ministry of Education), School of Geographical Sciences, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China.
Key Laboratory of Geographic Information Science (Ministry of Education), School of Geographical Sciences, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China; State Key Laboratory of Estuarine and Coastal Research, East China Normal University, 3663 North Zhongshan Road, Shanghai 200062, China.
Sci Total Environ. 2019 Jan 15;648:306-314. doi: 10.1016/j.scitotenv.2018.08.162. Epub 2018 Aug 14.
In the contaminated coastal sediments, variations of microbial community can reflect the impact of anthropogenic activities. The identification, evaluation and monitoring of the potential bio-indicator species and biomarker communities are vital for the ecological studies in sedimentary environments. Based on the high-throughput sequencing, the microbial communities were characterized in the sediments along the Yangtze Estuary and its coastal area. The results showed that the structure and composition of microbial communities varied greatly among different sampling sites at the phyla level, especially for Euryarchaeota. Metabolic pathway and quantitative PCR analyses suggested that the methane metabolism-related microbes were mainly included in the phylum of Euryarchaeota. Elevated abundances of methane metabolism-related microbes were found at Shidongkou (SDK) and Wusongkou (WSK), where microbes were seriously impacted by the wastewater treatment plant (WWTP) effluent and urban runoff. By comparing with the Euryarchaeota in WWTP sludge, the relatively high abundance of Euryarchaeota in sediment at SDK may be mainly related to the massive growth of indigenous species, promoted by anthropogenic nutrients. Moreover, redundancy discriminant analysis and correlation analysis revealed that methanogens and methanotrophs mainly respond to the nutrients and metals, such as total organic carbon, total phosphorus, total nitrogen, SO, NO, NH, Cr, and Zn, which were often related to human activities. Network analyses showed that the species related to the metabolism of methane may play a vital role in the interassociation among different microbial communities. Therefore, methanogens, methanotrophs and their community compositions could be considered as potential bio-indicator species and biomarker communities, indicating anthropogenic activities in the sediments along the Yangtze Estuary and its coastal area.
在受污染的沿海沉积物中，微生物群落的变化可以反映人为活动的影响。鉴定、评估和监测潜在的生物指示物种和生物标志物群落，对于沉积环境的生态研究至关重要。本研究基于高通量测序，对长江口及其沿海地区沉积物中的微生物群落进行了特征描述。结果表明，在门水平上，不同采样点的微生物群落结构和组成差异很大，尤其是古菌。代谢途径和定量 PCR 分析表明，甲烷代谢相关的微生物主要属于古菌门。在受到污水处理厂（WWTP）污水和城市径流严重影响的石洞口（SDK）和吴淞口（WSK），发现甲烷代谢相关微生物的丰度升高。与 WWTP 污泥中的古菌相比，SDK 沉积物中古菌的相对高丰度可能主要与土著物种的大量生长有关，这是由人为营养物质促进的。此外，冗余判别分析和相关分析表明，产甲烷菌和甲烷氧化菌主要响应总有机碳、总磷、总氮、SO、NO、NH、Cr 和 Zn 等与人类活动相关的营养物质和金属。网络分析表明，与甲烷代谢相关的物种可能在不同微生物群落之间的相互关联中起关键作用。因此，产甲烷菌、甲烷氧化菌及其群落组成可以作为潜在的生物指示物种和生物标志物群落，指示长江口及其沿海地区沉积物中的人为活动。
