School of Ocean, Yantai University, 30 QingQuan Road, Yantai, 264005, China.
Antonie Van Leeuwenhoek. 2023 Dec;116(12):1337-1344. doi: 10.1007/s10482-023-01890-5. Epub 2023 Oct 13.
In this study, we reported a Gram-stain-negative, rod-shaped, atrichous, and aerobic bacterial strain named YMD87, which was isolated from the intertidal zone sediment of Chinese Yellow Sea. Growth of strain YMD87 occurred at 10.0-40.0 °C (optimum, 25-30 °C), pH 4.0-12.0 (optimum, 8.0) and with 0-6.0% (w/v) NaCl (optimum, 0.0-2.0%). Phylogenetic tree analysis based on 16S rRNA gene sequence indicated that strain YMD87 belonged to the genus Tropicibacter and was closely related to Tropicibacter alexandrii LMIT003 (97.2% sequence similarity). Genomic analysis indicated that strain YMD87 contains a circular chromosome of 3,932,460 bp with G + C content of 63.8% and three circular plasmids of 116,492 bp, 49,209 bp and 49,673 bp, with G + C content of 64.3%. Genomic functional analysis revealed that strain YMD87T is potential a novel sulfur-metabolizing bacteria. The predominant respiratory quinone of YMD87 was ubiquinone-10 (Q-10). The major polar lipids of YMD87 contained phosphatidylglycerol, phosphatidylethanolamine, five unidentified lipids, five unidentified phospholipids, phosphatidylcholine, unidentified glycolipid and five unidentified aminolipids. The major fatty acids of strain YMD87 contained C 3-OH, C, and summed feature 8 (C18:1 ω7c or/and C18:1 ω6c). Phylogenetic, physiological, biochemical and morphological analyses suggested that strain YMD87 represents a novel species of the genus Tropicibacter, and the name Tropicibacter oceani sp. nov is proposed. The type strain is YMD87 (= MCCC 1K08473 = KCTC 92856).
在本研究中，我们报道了一株革兰氏阴性、杆状、无枝和需氧细菌，命名为 YMD87，它从中国黄海潮间带沉积物中分离得到。菌株 YMD87 的生长温度为 10.0-40.0°C（最佳温度为 25-30°C），pH 值为 4.0-12.0（最佳值为 8.0），盐度为 0-6.0%（w/v）（最佳值为 0.0-2.0%）。基于 16S rRNA 基因序列的系统发育树分析表明，菌株 YMD87 属于 Tropicibacter 属，与 Tropicibacter alexandrii LMIT003（序列相似度为 97.2%）密切相关。基因组分析表明，菌株 YMD87 含有一个大小为 3,932,460bp 的圆形染色体，G+C 含量为 63.8%，以及三个大小分别为 116,492bp、49,209bp 和 49,673bp 的圆形质粒，G+C 含量分别为 64.3%。基因组功能分析表明，菌株 YMD87T 是一种潜在的新型硫代谢细菌。YMD87 的主要呼吸醌为泛醌-10（Q-10）。YMD87 的主要极性脂包括磷脂酰甘油、磷脂酰乙醇胺、五种未鉴定的脂类、五种未鉴定的磷脂、磷脂酰胆碱、未鉴定的糖脂和五种未鉴定的氨基脂。菌株 YMD87 的主要脂肪酸包括 C3-OH、C16:0 和特征性脂肪酸 8（C18:1 ω7c 和/或 C18:1 ω6c）。系统发育、生理、生化和形态分析表明，菌株 YMD87 代表 Tropicibacter 属的一个新种，现提议命名为海洋 Tropicibacter 新种。模式菌株为 YMD87（=MCCC 1K08473=KCTC 92856）。
