Solopova Ana, Bachmann Herwig, Teusink Bas, Kok Jan, Kuipers Oscar P
Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, Netherlands.
Faculty of Earth and Life Sciences, Systems Bioinformatics IBIVU/NISB, Vrije Universiteit Amsterdam, Amsterdam, Netherlands.
Front Microbiol. 2018 Aug 3;9:1803. doi: 10.3389/fmicb.2018.01803. eCollection 2018.
Since the 1970s, galactose metabolism in has been in debate. Different studies led to diverse outcomes making it difficult to conclude whether galactose uptake was PEP- or ATP- dependent and decide what the exact connection was between galactose and lactose uptake and metabolism. It was shown that some strains possess two galactose-specific systems - a permease and a PTS, even if they lack the lactose utilization plasmid, proving that a lactose-independent PTS exists. However, the PTS transporter was never identified. Here, with the help of transcriptome analyses and genetic knock-out mutants, we reveal the identities of two low-affinity galactose PTSs. A novel plant-niche-related PTS component Llmg_0963 forming a hybrid transporter Llmg_0963PtcBA and a glucose/mannose-specific PTS are shown to be involved in galactose transport in MG1363.
自20世纪70年代以来，[具体生物名称]中的半乳糖代谢一直存在争议。不同的研究得出了不同的结果，因此很难确定半乳糖摄取是依赖磷酸烯醇式丙酮酸（PEP）还是依赖三磷酸腺苷（ATP），也难以确定半乳糖与乳糖摄取及代谢之间的确切联系。研究表明，一些[具体生物名称]菌株拥有两个半乳糖特异性系统——一种通透酶和一种磷酸转移酶系统（PTS），即使它们缺乏乳糖利用质粒，这证明存在不依赖乳糖的PTS。然而，从未鉴定出PTS转运体。在此，借助转录组分析和基因敲除突变体，我们揭示了两种低亲和力半乳糖PTS的身份。一种与植物生态位相关的新型PTS组分Llmg_0963形成了一种杂交转运体Llmg_0963PtcBA，并且一种葡萄糖/甘露糖特异性PTS被证明参与了[具体生物名称]MG1363中的半乳糖转运。
