Vahdat Sadaf, Pahlavan Sara, Aghdami Nasser, Bakhshandeh Behnaz, Baharvand Hossein
Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran.
Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
Cell J. 2019 Jan;20(4):496-504. doi: 10.22074/cellj.2019.5661. Epub 2018 Dec 12.
Cardiovascular progenitor cells (CPCs) are introduced as one of the promising cell sources for preclinical studies and regenerative medicine. One of the earliest type of CPCs is cardiogenic mesoderm cells (CMCs), which have the capability to generate all types of cardiac lineage derivatives. In order to benefit from CMCs, development of an efficient culture strategy is required. We aim to explore an optimized culture condition that uses human embryonic stem cell (hESC)-derived CMCs.
In this experimental study, hESCs were expanded and induced toward cardiac lineage in a suspension culture. Mesoderm posterior 1-positive (MESP1+) CMCs were subjected to four different culture conditions: i. Suspension culture of CMC spheroids, ii. Adherent culture of CMC spheroids, iii. Adherent culture of single CMCs using gelatin, and iv. Adherent culture of single CMCs using Matrigel.
Although, we observed no substantial changes in the percentage of MESP1+ cells in different culture conditions, there were significantly higher viability and total cell numbers in CMCs cultured on Matrigel (condition iv) compared to the other groups. CMCs cultivated on Matrigel maintained their progenitor cell signature, which included the tendency for cardiogenic differentiation.
These results showed the efficacy of an adherent culture on Matrigel for hESC-derived CMCs, which would facilitate their use for future applications.
心血管祖细胞（CPCs）被认为是临床前研究和再生医学中一种有前景的细胞来源。最早类型的CPCs之一是心脏中胚层细胞（CMCs），它有能力产生所有类型的心脏谱系衍生物。为了利用CMCs，需要开发一种有效的培养策略。我们旨在探索一种使用人胚胎干细胞（hESC）来源的CMCs的优化培养条件。
在本实验研究中，hESCs在悬浮培养中进行扩增并诱导向心脏谱系分化。中胚层后部1阳性（MESP1+）的CMCs被置于四种不同的培养条件下：i. CMC球体的悬浮培养；ii. CMC球体的贴壁培养；iii. 使用明胶对单个CMC进行贴壁培养；iv. 使用基质胶对单个CMC进行贴壁培养。
尽管我们观察到在不同培养条件下MESP1+细胞的百分比没有实质性变化，但与其他组相比，在基质胶上培养的CMCs（条件iv）具有显著更高的活力和总细胞数。在基质胶上培养的CMCs保持了它们的祖细胞特征，包括向心脏分化的倾向。
这些结果表明了使用基质胶对hESC来源的CMCs进行贴壁培养的有效性，这将有助于它们在未来的应用。
