通过DNA序列分析鉴定并定位九个T5噬菌体tRNA基因
Identification and location of nine T5 bacteriophage tRNA genes by DNA sequence analysis.
作者信息
Desai S M, Vaughan J, Weiss S B
出版信息
Nucleic Acids Res. 1986 May 27;14(10):4197-205. doi: 10.1093/nar/14.10.4197.
Abstract
Sequence analysis of two DNA fragments generated from bacteriophage T5 DNA by restriction with Hpa I and Hae III has resulted in the detection and localization of nine tRNA genes (His, two Ser genes, Leu, Val, Lys, fMet, Pro, and Ile). The genes which code for tRNAs His and Leu are partials, whereas the remaining genes are complete. A majority of the tRNA genes are located in close proximity to one another. A unique feature of the Pro and Ile genes is that their DNA sequence overlap.
摘要
通过用Hpa I和Hae III进行限制性内切从噬菌体T5 DNA产生的两个DNA片段的序列分析，已检测到九个tRNA基因（His、两个Ser基因、Leu、Val、Lys、fMet、Pro和Ile）并确定了其位置。编码tRNA His和Leu的基因是部分基因，而其余基因是完整的。大多数tRNA基因彼此紧邻。Pro和Ile基因的一个独特特征是它们的DNA序列重叠。
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