两种噬菌体T5丝氨酸转运RNA基因的克隆与DNA序列分析

Cloning and DNA sequence of the genes for two bacteriophage T5 tRNAsSer.

作者信息

Kryukov V M, Ksenzenko V N, Kaliman A V, Bayev A A

出版信息

FEBS Lett. 1983 Jul 11;158(1):123-7. doi: 10.1016/0014-5793(83)80690-5.

Abstract

Bacteriophage T5 Bg/II/Hind III DNA fragment (803 basepairs), containing the genes for 2 tRNAs and 2 RNAs with unknown functions, was cloned in the plasmid pBR322. The analysis of DNA sequence indicates that tRNA genes code isoacceptor tRNAsSer (tRNA1Ser and tRNA2Ser) with anticodons UGA and GGA, respectively. The main unusual structural feature of these tRNAs is the presence of extra non-basepaired nucleotides in the joinings of stem 'b' with stems 'a' and 'c'.

摘要

含有2个tRNA基因和2个功能未知的RNA基因的噬菌体T5 Bg/II/Hind III DNA片段（803个碱基对）被克隆到质粒pBR322中。DNA序列分析表明，tRNA基因分别编码反密码子为UGA和GGA的同功受体tRNAsSer（tRNA1Ser和tRNA2Ser）。这些tRNA的主要异常结构特征是在茎“b”与茎“a”和“c”的连接处存在额外的非碱基配对核苷酸。