两种噬菌体T5丝氨酸转运RNA基因的克隆与DNA序列分析

Cloning and DNA sequence of the genes for two bacteriophage T5 tRNAsSer.

作者信息

Kryukov V M, Ksenzenko V N, Kaliman A V, Bayev A A

出版信息

FEBS Lett. 1983 Jul 11;158(1):123-7. doi: 10.1016/0014-5793(83)80690-5.

DOI:10.1016/0014-5793(83)80690-5

Abstract

Bacteriophage T5 Bg/II/Hind III DNA fragment (803 basepairs), containing the genes for 2 tRNAs and 2 RNAs with unknown functions, was cloned in the plasmid pBR322. The analysis of DNA sequence indicates that tRNA genes code isoacceptor tRNAsSer (tRNA1Ser and tRNA2Ser) with anticodons UGA and GGA, respectively. The main unusual structural feature of these tRNAs is the presence of extra non-basepaired nucleotides in the joinings of stem 'b' with stems 'a' and 'c'.

摘要

含有2个tRNA基因和2个功能未知的RNA基因的噬菌体T5 Bg/II/Hind III DNA片段（803个碱基对）被克隆到质粒pBR322中。DNA序列分析表明，tRNA基因分别编码反密码子为UGA和GGA的同功受体tRNAsSer（tRNA1Ser和tRNA2Ser）。这些tRNA的主要异常结构特征是在茎“b”与茎“a”和“c”的连接处存在额外的非碱基配对核苷酸。

相似文献

1

Cloning and DNA sequence of the genes for two bacteriophage T5 tRNAsSer.两种噬菌体T5丝氨酸转运RNA基因的克隆与DNA序列分析

FEBS Lett. 1983 Jul 11;158(1):123-7. doi: 10.1016/0014-5793(83)80690-5.

2

Nucleotide sequence of the bacteriophage T5 DNA fragment which contains the gene for tRNAAsp.

Eur J Biochem. 1986 Apr 15;156(2):285-9. doi: 10.1111/j.1432-1033.1986.tb09579.x.

3

Cloning of genes for bacteriophage T5 stable RNAs.噬菌体T5稳定RNA基因的克隆

Biochim Biophys Acta. 1982 May 31;697(2):235-42. doi: 10.1016/0167-4781(82)90082-3.

4

The nucleotide sequence of bacteriophage T5 leucine tRNA.噬菌体T5亮氨酸转运核糖核酸的核苷酸序列。

FEBS Lett. 1985 Nov 18;192(2):299-302. doi: 10.1016/0014-5793(85)80129-0.

5

The nucleotide sequence of bacteriophage T5 glutamine transfer RNA.

Biochim Biophys Acta. 1984 Jul 18;782(3):313-9. doi: 10.1016/0167-4781(84)90067-8.

6

[Cloning of bacteriophage T5 DNA fragments in the plasmid pBR322. Analysis of recombinant plasmids by the method of bonding with RNA-polymerase from Escherichia coli on nitrocellulose filters].[噬菌体T5 DNA片段在质粒pBR322中的克隆。通过在硝酸纤维素滤膜上与大肠杆菌RNA聚合酶结合的方法分析重组质粒]

Mol Biol (Mosk). 1982 Nov-Dec;16(6):1253-62.

7

Cloning of bacteriophage T5 DNA fragments in plasmid pBR322 and bacteriophage lambda gtWES.噬菌体T5 DNA片段在质粒pBR322和噬菌体λgtWES中的克隆。

Gene. 1979 Dec;8(1):53-68. doi: 10.1016/0378-1119(79)90007-6.

8

Nucleotide sequence of the bacteriophage T4 gene 57 and a deduced amino acid sequence.噬菌体T4基因57的核苷酸序列及推导的氨基酸序列。

Nucleic Acids Res. 1982 Feb 11;10(3):1105-12. doi: 10.1093/nar/10.3.1105.

9

Hybrid transfer RNA genes in phage T4.

Cell. 1984 Aug;38(1):225-31. doi: 10.1016/0092-8674(84)90544-0.

10

Cloning of the bacteriophage T7 lysozyme gene.噬菌体T7溶菌酶基因的克隆

Chin J Biotechnol. 1990;6(2):87-93.

引用本文的文献

1

Exploiting tRNAs to Boost Virulence.利用转运RNA增强毒力。

Life (Basel). 2016 Jan 19;6(1):4. doi: 10.3390/life6010004.

2

Nucleotide sequence of the bacteriophage T5 DNA fragment containing a distal part of tRNA gene region.包含tRNA基因区域远端部分的噬菌体T5 DNA片段的核苷酸序列。

Nucleic Acids Res. 1987 Jul 10;15(13):5480-1. doi: 10.1093/nar/15.13.5480.

3

Identification and location of nine T5 bacteriophage tRNA genes by DNA sequence analysis.通过DNA序列分析鉴定并定位九个T5噬菌体tRNA基因

Nucleic Acids Res. 1986 May 27;14(10):4197-205. doi: 10.1093/nar/14.10.4197.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。