促肾上腺皮质激素1-4增强α-促黑素诱导的黑素细胞分散和过度梳理行为。
ACTH1-4 potentiates alpha-MSH-induced melanophore dispersion and excessive grooming.
作者信息
De Graan P N, Spruijt B M, Eberle A N, Girard J, Gispen W H
出版信息
Peptides. 1986 Jan-Feb;7(1):1-4. doi: 10.1016/0196-9781(86)90051-3.
The biological activity and a possible modulatory role of the N-terminal tetrapeptide Ser-Tyr-Ser-Met from alpha-MSH/ACTH was tested in the Anolis melanophore assay, the Xenopus melanophore assay, tyrosinase stimulation in mouse melanoma cells and in excessive grooming in the rat. ACTH1-4 did not exhibit biological activity in any of these four assays nor did it have modulatory properties in the Xenopus and the melanoma cell assay. However, in the Anolis assay ACTH1-4 potentiated pigment dispersion induced by alpha-MSH, alpha-MSH5-13 and ACTH1-24 by a factor of about 2. In the grooming assay ACTH1-4 potentiated the effects of alpha-MSH, alpha-MSH5-13, ACTH1-16 and ACTH5-16, but not those of ACTH1-24. Oxidized ACTH1-4 was without biological activity and potentiating properties in all four assays. This study shows that small fragments of the pro-opiomelanocortin precursor, which are devoid of biological activity, can modulate peripheral and central actions of alpha-MSH/ACTH.
对源自α-促黑素细胞激素/促肾上腺皮质激素（α-MSH/ACTH）的N端四肽丝氨酸-酪氨酸-丝氨酸-蛋氨酸（Ser-Tyr-Ser-Met）的生物活性及其可能的调节作用，在绿安乐蜥黑素细胞试验、非洲爪蟾黑素细胞试验、小鼠黑色素瘤细胞中的酪氨酸酶刺激试验以及大鼠过度梳理试验中进行了测试。促肾上腺皮质激素1-4（ACTH1-4）在这四种试验中的任何一种中均未表现出生物活性，在非洲爪蟾和黑色素瘤细胞试验中也没有调节特性。然而，在绿安乐蜥试验中，ACTH1-4使由α-MSH、α-MSH5-13和ACTH1-24诱导的色素扩散增强了约2倍。在梳理试验中，ACTH1-4增强了α-MSH、α-MSH5-13、ACTH1-16和ACTH5-16的作用，但没有增强ACTH1-24的作用。氧化型ACTH1-4在所有四种试验中均无生物活性和增强特性。这项研究表明，阿片-黑素细胞皮质素原前体的小片段虽无生物活性，但可调节α-MSH/ACTH的外周和中枢作用。
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