Trovato L, Scalia G, Palermo C I, Costanzo C M, Oliveri S
U.O.C. Laboratory Analysis Unit, A.O.U. "Policlinico-Vittorio Emanuele," Catania.
Department of Biomedical and Biotechnological Sciences, University of Catania, Catania, Italy.
Med Mycol. 2019 Jun 1;57(4):429-433. doi: 10.1093/mmy/myy071.
Isavuconazole is a new triazole with an expanded-spectrum and potent activity against moulds and yeasts. It has been authorized for use in adults for the treatment of invasive aspergillosis and for mucormycosis. The only commercially available isavuconazole susceptibility test is the minimum inhibitory concentration (MIC) strip isavuconazole test. The objective of this study was to assess the in vitro activity of isavuconazole using gradient concentration MIC strips, compared with the EUCAST broth microdilution reference method. A total of 147 clinically relevant fungal isolates comprising 120 Aspergillus sp. and 27 Scedosporium apiospermum complex were tested for susceptibility to isavuconazole using the EUCAST broth microdilution method and by the MIC strip isavuconazole test. The percent essential agreement between the two methods was calculated within a 1-fold dilution. The geometric means for the MICs using the EUCAST reference methods and the strip test were respectively: 0.60 mg/l and 0.65 mg/l for A. fumigatus, 0.70 mg/l and 0.77 mg/l for A. flavus, 1.50 mg/l and 1.25 mg/l for A. niger, 0.41 mg/l and 0.38 mg/l for A. terreus, 1.22 mg/l and 1.08 mg/l for S. apiospermum complex. The isavuconazole MIC strips showed good agreement with the EUCAST reference method. Isavuconazole MIC strips could be useful for susceptibility testing of Aspergillus sp. and S. apiospermum complex.
艾沙康唑是一种新型三唑类药物，具有广谱活性，对霉菌和酵母菌有强大的抗菌作用。它已被批准用于成人侵袭性曲霉病和毛霉病的治疗。唯一可商购的艾沙康唑药敏试验是最低抑菌浓度（MIC）条带法艾沙康唑试验。本研究的目的是使用梯度浓度MIC条带法评估艾沙康唑的体外活性，并与欧洲抗菌药物敏感性试验委员会（EUCAST）肉汤微量稀释参考方法进行比较。使用EUCAST肉汤微量稀释法和MIC条带法艾沙康唑试验，对总共147株临床相关真菌分离株进行了艾沙康唑药敏试验，其中包括120株曲霉属菌株和27株尖端赛多孢菌复合体菌株。两种方法之间的基本一致率在1倍稀释范围内计算。使用EUCAST参考方法和条带试验的MIC几何平均值分别为：烟曲霉为0.60mg/L和0.65mg/L，黄曲霉为0.70mg/L和0.77mg/L，黑曲霉为1.50mg/L和1.25mg/L，土曲霉为0.41mg/L和0.38mg/L，尖端赛多孢菌复合体为1.22mg/L和1.08mg/L。艾沙康唑MIC条带法与EUCAST参考方法显示出良好的一致性。艾沙康唑MIC条带法可用于曲霉属菌株和尖端赛多孢菌复合体的药敏试验。
