Arendrup Maiken Cavling, Verweij Paul, Nielsen Henrik Vedel
Unit of Mycology, Statens Serum Institut, Copenhagen, Denmark
Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark.
Antimicrob Agents Chemother. 2016 Dec 27;61(1). doi: 10.1128/AAC.01659-16. Print 2017 Jan.
We evaluated the MIC Strip Isavuconazole test against EUCAST E.Def 9.3 by using 40 wild-type and 39 CYP51A mutant Aspergillus fumigatus strains. The strip full inhibition endpoint (FIE) and 80% growth inhibition endpoint were determined by two independent readers, reader 1 (R1) and R2. The essential (within ±0, ±1, and ±2 twofold dilutions) and categorical agreements were best with the FIE (for R1/R2, 42%/41%, 75%/73%, and 90%/89% for essential agreement, and 91.1%/92.4% categorical agreement, with 6.3/8.9% very major errors and 0/1.3% major errors, respectively). The MIC Strip Isavuconazole test with the FIE appears to be useful.
我们使用40株野生型和39株CYP51A突变型烟曲霉菌株，针对欧盟CAST E.Def 9.3评估了MIC条带伊曲康唑测试。条带完全抑制终点（FIE）和80%生长抑制终点由两名独立的阅片者，即阅片者1（R1）和阅片者2（R2）确定。基本一致性（在±0、±1和±2倍稀释范围内）和分类一致性在FIE时最佳（对于R1/R2，基本一致性分别为42%/41%、75%/73%和90%/89%，分类一致性为91.1%/92.4%，非常重大误差分别为6.3/8.9%，重大误差分别为0/1.3%）。采用FIE的MIC条带伊曲康唑测试似乎很有用。
