Lo Cascio Giuliana, Bazaj Alda, Trovato Laura, Sanna Silvana, Andreoni Stefano, Blasi Elisabetta, Conte Marco, Fazii Paolo, Oliva Ester, Lepera Valentina, Lombardi Gianluigi, Farina Claudio
Clinical Microbiology and Virology Unit, Azienda Ospedaliera Universitaria Integrata, Verona, Italy.
Medical Mycology Committee, Italian Society of Clinical Microbiologist, Milan, Italy.
Infect Drug Resist. 2022 Oct 4;15:5839-5848. doi: 10.2147/IDR.S367082. eCollection 2022.
In this study the activity of Isavuconazole, Voriconazole, Amphotericin B, and Caspofungin against 224 clinical isolates of spp. originating from seven Italian hospitals, was comparatively evaluated with two commercial antifungal susceptibility tests (AST): Sensititre YeastOne (SYO) and MIC Test Strip. More attention was focused on Isavuconazole activity, given the new introduction of the drug in widely distributed antifungal susceptibilities methods in the clinical microbiology lab. The minimum inhibitory concentrations of antifungal drug that can inhibit the growth of pathogen by 90% (MIC90) for Isavuconazole detected by SYO were 0.5, 1, 0.25, and 2 µg/mL for , , , and , respectively, whilst they were 0.25, 0.25, 0.5, and 0.75 µg/mL by MIC Test Strip. Essential agreement between the two tested methods for Isavuconazole is 70% for all the species tested, 75.7% for , 45.2% for , 90.6% for . , and 40% for . Although the tested strains do not express any phenotypic resistance, MIC results were quite different if tested with microdilution broth or gradient agar method. This is the first Italian multicenter report on Isavuconazole MIC obtained employing the widely used Sensititre Yeast One (SYO) and MIC Test Strip on clinical isolates of .
在本研究中，使用两种商业抗真菌药敏试验（AST）：Sensititre YeastOne（SYO）和MIC试纸条，对源自意大利七家医院的224株临床分离菌的伊曲康唑、伏立康唑、两性霉素B和卡泊芬净活性进行了比较评估。鉴于该药物新引入临床微生物实验室广泛使用的抗真菌药敏方法中，所以对伊曲康唑活性给予了更多关注。通过SYO检测，伊曲康唑对、、、和的能抑制病原体生长90%的最低抑菌浓度（MIC90）分别为0.5、1、0.25和2μg/mL，而通过MIC试纸条检测时分别为0.25、0.25、0.5和0.75μg/mL。两种检测方法对伊曲康唑的基本一致性，对于所有测试菌种为70%，对于为75.7%，对于为45.2%，对于为90.6%，对于为40%。尽管测试菌株未表现出任何表型抗性，但如果用微量稀释肉汤法或梯度琼脂法测试，MIC结果差异很大。这是意大利第一份关于使用广泛应用的Sensititre Yeast One（SYO）和MIC试纸条对临床分离菌进行伊曲康唑MIC检测的多中心报告。
