Department of Adult Neurology, Medical University of Gdańsk & University Clinical Centre, Poland.
Department of Clinical Immunology and Transplantology, Medical University of Gdańsk, Poland.
Neurochem Int. 2018 Nov;120:134-139. doi: 10.1016/j.neuint.2018.08.009. Epub 2018 Aug 17.
Stroke causes an efflux of various groups of progenitor/stem cells from bone marrow to bloodstream and a rise in neuron specific enolase (NSE) serum concentrations. The aim of this study was to identify activity of chosen stem/progenitor cells during first 7 days after stroke through correlations between these cells levels and NSE values. Additional goal was to confirm the role of NSE as a prognostic marker of ischemic stroke. Venous blood was collected repeatedly from 67 acute ischemic stroke patients and 15 control subjects, in order to assess NSE with ELISA, and CD45CD34 CD271+, CD45CD34 + CXCR4+, CD45CD34 + CXCR7+ and CD45CD34 CD133 + stem/progenitor cells by means of flow cytometry. Patients underwent repeated assessment with the National Ischemic Stroke Scale and modified Rankin Scale. Ischemic lesion volumes were assessed twice by MRI-DWI (day 1 and 5 ± 2). NSE correlated negatively with MFI levels of the CD45CD34 + CXCR7+ cells, and percentage levels of the CD45CD34 and CD45CD34 + CXCR4+ cells. NSE concentrations were significantly higher in patients compared to control subjects. NSE on day 2 positively correlated with lesion volume on both MRI. NSE on day 2 and 6-7 correlated positively with initial NIHSS scores, and on day 1 with mRS score on day 9. In conclusion, in this study NSE indicated some activity of the CD45CD34 + CXCR7+, CD45CD34 and CD45CD34 + CXCR4+ stem/progenitor cells in the first 7 days after ischemic stroke. Additionally, this study supports the thesis that NSE might be a valuable prognostic marker in acute ischemic stroke.
中风会导致各种祖细胞/干细胞从骨髓流出到血液中，并导致神经元特异性烯醇化酶（NSE）血清浓度升高。本研究的目的是通过这些细胞水平与 NSE 值之间的相关性，在中风后 7 天内确定选择的干细胞/祖细胞的活性。另一个目的是确认 NSE 作为缺血性中风预后标志物的作用。从 67 名急性缺血性中风患者和 15 名对照者中反复采集静脉血，以酶联免疫吸附试验（ELISA）评估 NSE，并通过流式细胞术评估 CD45CD34+CD271+、CD45CD34+CXCR4+、CD45CD34+CXCR7+和 CD45CD34+CD133+干细胞/祖细胞。患者接受了国家缺血性中风量表和改良 Rankin 量表的重复评估。通过 MRI-DWI 在第 1 天和第 5 天（±2 天）两次评估缺血性病变体积。NSE 与 CD45CD34+CXCR7+细胞的 MFI 水平呈负相关，与 CD45CD34+和 CD45CD34+CXCR4+细胞的百分比水平呈负相关。与对照组相比，NSE 在患者中的浓度明显更高。第 2 天的 NSE 与 MRI 上的病变体积呈正相关。第 2 天和第 6-7 天的 NSE 与初始 NIHSS 评分呈正相关，第 1 天的 NSE 与第 9 天的 mRS 评分呈正相关。总之，在本研究中，NSE 表明在缺血性中风后 7 天内，CD45CD34+CXCR7+、CD45CD34+和 CD45CD34+CXCR4+干细胞/祖细胞有一定的活性。此外，本研究支持 NSE 可能是急性缺血性中风有价值的预后标志物的观点。
