Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Biosens Bioelectron. 2018 Nov 15;119:176-181. doi: 10.1016/j.bios.2018.08.020. Epub 2018 Aug 11.
The accurate and rapid detection of cardiac troponin I (cTnI) at an early stage can prevent people from getting acute myocardial infarction to a great extent. Here, an ultrasensitive sandwich-type electrochemical immunosensor for the quantitative detection of cTnI is designed based on a novel signal amplification strategy. Gold nanoparticles (Au NPs) doped covalent organic frameworks (COFs) and electron mediator toluidine blue (TB-Au-COFs) behave as labels for achieving signal amplification. Note that polypyrrole modified titanium dioxide nanoparticles (TiO-PPy) with a high electronic conductivity and a large specific surface area can promote the performance of the immunosensor. For the immobilization of primary antibodies, Au NPs decorated TiO-PPy (TiO-PPy-Au) is used as the substrate material to improve electron transfer. The proposed method exhibits a linear range from 0.5 pg mL to 10.0 ng mL and a low detection limit of 0.17 pg mL (S/N = 3). The immunosensor exhibits good stability, acceptable reproducibility and accuracy, indicating potential applications in clinical diagnostics.
早期准确快速地检测心肌肌钙蛋白 I (cTnI)，可以在很大程度上预防人们患上急性心肌梗死。在这里，设计了一种基于新型信号放大策略的超灵敏夹心型电化学免疫传感器，用于定量检测 cTnI。金纳米粒子 (Au NPs) 掺杂共价有机框架 (COFs) 和电子媒介甲苯胺蓝 (TB-Au-COFs) 用作标签，以实现信号放大。值得注意的是，具有高导电性和大比表面积的聚吡咯修饰的二氧化钛纳米粒子 (TiO-PPy) 可以促进免疫传感器的性能。为了固定主要抗体，使用 Au NPs 修饰的 TiO-PPy (TiO-PPy-Au) 作为基底材料，以促进电子转移。所提出的方法在 0.5 pg mL 至 10.0 ng mL 的线性范围内表现出良好的线性，检测限低至 0.17 pg mL (S/N = 3)。该免疫传感器表现出良好的稳定性、可接受的重现性和准确性，表明其在临床诊断中有潜在的应用。
