Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Yunnan Province Key Lab of Wood Adhesives and Glued Products, School of Materials Science and Engineering, Southwest Forestry University, Kunming 650224, China; School of Chemical Science and Technology, Yunnan University, Kunming 650091, China.
Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Yunnan Province Key Lab of Wood Adhesives and Glued Products, School of Materials Science and Engineering, Southwest Forestry University, Kunming 650224, China.
Biosens Bioelectron. 2019 Apr 1;130:214-224. doi: 10.1016/j.bios.2019.01.041. Epub 2019 Jan 29.
In this work, a novel, facile, clean synthesis of monodisperse Au nanoparticles (AuNPs) with an average diameter of 5 nm was achieved by reducing HAuCl with dihydroxylatopillar[5]arene (2HP5) in basic solution without the use of harsh reagents and/or external energy. Accordingly, toluidine blue (TB), one electrochemcial indicator, could enter into the cavity of 2HP5 to fabricate host-guest complex through strong electrostatic interaction and charge-transfer interaction, which significantly enhanced the loading quantity of TB and effectively suppressed the leaking of TB resulting in an ultrasensitive and robust electrochemical response. More importantly, the integration of 2HP5-stabilized AuNPs and Pd-decorated MnO nanocomposites (2HP5@Au-Pd/MnO) might usually obtain a novel functional-enhanced materials and lead to new properties and improving the analytical performance and robustness of electrochemical devices. Therefore, we construct a sandwich-type electrochemical immunosensor using TB-2HP5@Au-Pd/MnO nanocomposites as the transducing materials for robust and ultrasensitive detection of cardiac troponin I (cTnI), a significant biomarker of acute myocardial infarction. As anticipated, this immunosensor had remarkable robustness, sensitivity, stability, specificity, and corresponded linearly to the concentration of cTnI over a wide range from 0.005 to 20 ng mL with a low detection limit of 2 pg mL (S/N = 3). The proposed electrochemical immunosensor showed acceptable recoveries in human serum, indicating that macrocycle-stabilized metal nanoparticle might be a promising emerging transducer material for the detection of biological markers.
在这项工作中，通过在碱性溶液中用二羟基柱[5]芳烃（2HP5）还原 HAuCl，实现了平均直径为 5nm 的单分散金纳米粒子（AuNPs）的新颖、简便、清洁合成，而无需使用苛刻的试剂和/或外部能量。因此，电化学指示剂甲苯胺蓝（TB）可以通过强静电相互作用和电荷转移相互作用进入 2HP5 的空腔，从而制备主体-客体配合物，这显著增加了 TB 的负载量，并有效地抑制了 TB 的泄漏，从而产生了超灵敏和稳健的电化学响应。更重要的是，2HP5 稳定的 AuNPs 和 Pd 修饰的 MnO 纳米复合材料（2HP5@Au-Pd/MnO）的整合通常可以获得新型功能增强材料，并导致新的性质，提高电化学器件的分析性能和稳健性。因此，我们使用 TB-2HP5@Au-Pd/MnO 纳米复合材料作为信号转导材料构建了三明治型电化学免疫传感器，用于稳健和超灵敏检测心肌肌钙蛋白 I（cTnI），这是急性心肌梗死的一个重要生物标志物。正如预期的那样，该免疫传感器具有显著的稳健性、灵敏度、稳定性、特异性，并且在 0.005 至 20ngmL 的宽浓度范围内与 cTnI 的浓度呈线性关系，检测限低至 2pgmL（S/N=3）。该电化学免疫传感器在人血清中的回收率可接受，表明大环稳定的金属纳米粒子可能是用于检测生物标志物的有前途的新兴传感器材料。
