Department of Tobacco, College of Tobacco, Henan Agricultural University, Zhengzhou 450002, Henan, China.
Nanyang Branch of Henan Province Tobacco Company, Nanyang 473003, Henan, China.
Molecules. 2018 Aug 20;23(8):2081. doi: 10.3390/molecules23082081.
Root-knot nematodes are one of the most destructive pathogens, causing severe losses to tobacco productivity and quality. However, the underlying resistance mechanism of tobacco to is not clear. In this study, two tobacco genotypes, K326 and Changbohuang, which are resistant and susceptible to , respectively, were used for RNA-sequencing analysis. An average of 35 million clean reads were obtained. Compared with their expression levels in non-infected plants of the same genotype, 4354 and 545 differentially expressed genes (DEGs) were detected in the resistant and susceptible genotype, respectively, after invasion. Overall, 291 DEGs, involved in diverse biological processes, were common between the two genotypes. Genes encoding toxic compound synthesis, cell wall modification, reactive oxygen species and the oxidative burst, salicylic acid signal transduction, and production of some other metabolites were putatively associated with tobacco resistance to . In particular, the complex resistance response needed to overcome invasion may be regulated by several transcription factors, such as the ethylene response factor, MYB, basic helix⁻loop⁻helix transcription factor, and indole acetic acid⁻leucine-resistant transcription factor. These results may aid in the identification of potential genes of resistance to for tobacco cultivar improvement.
根结线虫是最具破坏性的病原体之一，对烟草的生产力和质量造成严重损失。然而，烟草对根结线虫的潜在抗性机制尚不清楚。在这项研究中，使用了对根结线虫具有抗性和敏感性的两个烟草基因型 K326 和 Changbohuang 进行 RNA 测序分析。平均获得了 3500 万条清洁reads。与相同基因型的未感染植物相比，在抗性和敏感性基因型中分别检测到 4354 个和 545 个差异表达基因（DEGs）。总体而言，291 个 DEGs 参与了不同的生物过程，在两个基因型之间是共同的。编码有毒化合物合成、细胞壁修饰、活性氧和氧化爆发、水杨酸信号转导以及产生其他一些代谢物的基因被推测与烟草对根结线虫的抗性有关。特别是，克服根结线虫入侵所需的复杂抗性反应可能受到几个转录因子的调节，如乙烯反应因子、MYB、碱性螺旋-环-螺旋转录因子和吲哚乙酸-亮氨酸抗性转录因子。这些结果可能有助于鉴定烟草品种改良的潜在抗性基因。
